Characterization of ten microsatellite loci in the blue mussel Mytilus edulis

Type Article
Date 2009-08
Language English
Author(s) Lallias Delphine1, Stockdale Ruth1, Boudry PierreORCID2, Lapegue SylvieORCID3, Beaumont Andy R.1
Affiliation(s) 1 : Bangor Univ, Coll Nat Sci, Ctr Appl Marine Sci, Sch Ocean Sci, Menai Bridge LL59 5AB, Gwynedd, Wales.
2 : IFREMER, UMR Physiol & Ecophysiol Mollusques Marins M100, Plouzane, France.
3 : IFREMER, Lab Genet & Pathol, F-17390 Ronce Les Bains, La Tremblade, France.
Source Journal Of Shellfish Research (0730-8000) (National Shellfisheries Association), 2009-08 , Vol. 28 , N. 3 , P. 547-551
WOS© Times Cited 19
Keyword(s) Mytilus edulis, microsatellites, universal tailed-primer labeling, null alleles, mussels
Abstract Mussels of the genus Mytilus are ecologically and commercially important worldwide, and they form hybrid complexes where their distributions overlap. Allozyme and nuclear markers have bee It Used to investigate their genetics over many years, but successful development of reliable highly valuable microsatellite markers has lagged be hind other shellfish species. We have developed and characterized tell novel microsatellite loci that amplify reliably for the blue mussel Mytilus edulis. The number of alleles among 30 individuals from a wild population (Menai Strait, North Wales UK) ranged between 9 and 29 and the observed heterozygosity between 0.300 and 0.954. Significant heterozygote deficiencies against the Hardy-Weinberg model were observed at 6 out of 10 loci. Analyses using MICRO-CHECKER suggested the presence of null alleles at 8 out of 10 microsatellites with estimated null allele frequencies ranging front 0.105-0.305. The 10 newly developed microsatellites will have value to discriminate between Mytilus species, to support studies of introgression and hybridization and to strengthen and improve the available genetic linkage map.
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Lallias Delphine, Stockdale Ruth, Boudry Pierre, Lapegue Sylvie, Beaumont Andy R. (2009). Characterization of ten microsatellite loci in the blue mussel Mytilus edulis. Journal Of Shellfish Research, 28(3), 547-551. Open Access version :