FN Archimer Export Format PT J TI Detection of Bonamia ostreae and B. exitiosa (Haplosporidia) in Ostrea edulis from the Adriatic Sea (Italy) BT AF NARDSI, V. ARZUL, Isabelle CARGINI, D. MOSCA, F. CALZETTA, A. TRAVERSA, D. ROBERT, Maeva JOLY, Jean-Pierre CHOLLET, Bruno RENAULT, Tristan TISCAR, P. G. AS 1:1;2:2;3:1;4:1;5:1;6:1;7:2;8:2;9:2;10:2;11:1; FF 1:;2:PDG-DOP-DCN-AGSAE-LGP;3:;4:;5:;6:;7:PDG-DOP-DCN-AGSAE-LGP;8:PDG-DOP-DCN-AGSAE-LGP;9:PDG-DOP-DCN-AGSAE-LGP;10:PDG-DOP-DCN-AGSAE-LGP;11:; C1 Univ Teramo, Dept Comparat Biomed Sci, I-64100 Teramo, Italy. IFREMER, Lab Genet & Pathol, F-17390 Ronce Les Bains, La Tremblade, France. C2 UNIV TERAMO, ITALY IFREMER, FRANCE SI LA TREMBLADE SE PDG-DOP-DCN-AGSAE-LGP IN WOS Ifremer jusqu'en 2018 copubli-europe IF 1.572 TC 37 UR https://archimer.ifremer.fr/doc/00002/11284/7825.pdf https://archimer.ifremer.fr/doc/00002/11284/11555.pdf LA English DT Article DE ;Bonamia spp.;Ostrea edulis;Molecular analysis;Electron microscopy AB The flat oyster Ostrea edulis L. is widespread along the Italian coasts. In particular, the Manfredonia Gulf (Adriatic Sea) represents an important site where natural beds subsist. Previous monitoring conducted in 1990 by light microscopy and ultrastructural studies revealed the presence of Bonamia-like microcell parasites in some flat oysters: following this observation, a new sampling of O. edulis was carried out at this location in 2007. Of 750 oysters collected, 3 showed the presence of uninucleated microcells (2 to 3 mu m diameter) free or inside the haemocyte cytoplasm by cytology and histopathology. Molecular analysis confirmed that the microcells in 2 oysters were B. exitiosa, whereas in the third oyster the microcells were B. ostreae. Moreover, molecular studies were carried out to confirm the existence of Bonamia sp. in archived samples, confirming the presence of B. ostreae in the Manfredonia Gulf since 1990. PY 2010 PD FEB SO Diseases Of Aquatic Organisms SN 0177-5103 PU Inter-research VL 89 IS 1 UT 000275737800009 BP 79 EP 85 DI 10.3354/dao02167 ID 11284 ER EF