Effects of Alexandrium minutum exposure upon physiological and hematological variables of diploid and triploid oysters, Crassostrea gigas

Type Article
Date 2010-04
Language English
Author(s) Haberkorn Hansy1, Lambert Christophe1, Le Goic Nelly1, Gueguen Marielle2, Moal Jeanne3, Palacios Elena4, Lassus Patrick2, Soudant Philippe1
Affiliation(s) 1 : Univ Bretagne Occidentale, Lab Sci Environm Marin, Inst Univ Europeen Mer, F-29280 Plouzane, France.
2 : IFREMER Ctr Nantes, Lab Phycotoxines, F-44311 Nantes, France.
3 : IFREMER, Ctr Brest, Lab Physiol Mollusques, F-29280 Plouzane, France.
4 : Ctr Invest Biol Noroeste CIBNOR, La Paz, Bcs, Mexico.
Source Aquatic Toxicology (0166-445X) (Elsevier Science Bv), 2010-04 , Vol. 97 , N. 2 , P. 96-108
DOI 10.1016/j.aquatox.2009.12.006
WOS© Times Cited 63
Keyword(s) Oysters, Ploidy, Physiology, Harmful-algal bloom, Alexandrium minutum, PST accumulation
Abstract The effects of an artificial bloom of the toxin-producing dinoflagellate, Alexandrium minutum, upon physiological parameters of the Pacific oyster, Crassostrea gigas, were assessed. Diploid and triploid oysters were exposed to cultured A. minutum and compared to control diploid and triploid oysters fed T. Isochrysis. Experiments were repeated twice, in April and mid-May 2007, to investigate effects of maturation stage on oyster responses to A. minutum exposure. Oyster maturation stage, Paralytic Shellfish Toxin (PST) accumulation, as well as several digestive gland and hematological variables, were assessed at the ends of the exposures. In both experiments, triploid oysters accumulated more PSTs (approximately twice) than diploid oysters. Significant differences, in terms of phenoloxidase activity (PO) and reactive oxygen species (ROS) production of hemocytes, were observed between A. minutum-exposed and non-exposed oysters. PO in hemocytes was lower in oysters exposed to A. minutum than in control oysters in an early maturation stage (diploids and triploids in April experiment and triploids in May experiment), but this contrast was reversed in ripe oysters (diploids in May experiment). In the April experiment, granulocytes of oysters exposed to A. minutum produced more ROS than those of control oysters; however, in the May experiment, ROS production of granulocytes was lower in A. minutum-exposed oysters. Moreover, significant decreases in free fatty acid, monoacylglycerol, and diacylglycerol contents in digestive glands of oysters exposed to A. minutum were observed. Concurrently, the ratio of reserve lipids (triacylglycerol, ether glycerides and sterol esters) to structural lipids (sterols) decreased upon A. minutum exposure in both experiments. Also, several physiological responses to A. minutum exposure appeared to be modulated by maturation stage as well as ploidy of the oysters. (C) 2009 Elsevier B.V. All rights reserved.
Full Text
File Pages Size Access
13 661 KB Access on demand
Author's final draft 28 172 KB Open access
Top of the page

How to cite 

Haberkorn Hansy, Lambert Christophe, Le Goic Nelly, Gueguen Marielle, Moal Jeanne, Palacios Elena, Lassus Patrick, Soudant Philippe (2010). Effects of Alexandrium minutum exposure upon physiological and hematological variables of diploid and triploid oysters, Crassostrea gigas. Aquatic Toxicology, 97(2), 96-108. Publisher's official version : https://doi.org/10.1016/j.aquatox.2009.12.006 , Open Access version : https://archimer.ifremer.fr/doc/00003/11378/