FN Archimer Export Format
PT J
TI Application of library-independent microbial source tracking methods for identifying the sources of faecal contamination in coastal areas
BT 
AF GOURMELON, Michele
   CAPRAIS, Marie-Paule
   LE MENNEC, Cecile
   MIESZKIN, Sophie
   PONTHOREAU, C.
   GENDRONNEAU, M.
AS 1:1;2:1;3:1;4:1;5:2;6:2;
FF 1:PDG-DOP-DCN-EMP-MIC;2:PDG-DOP-DCN-EMP-MIC;3:PDG-DOP-DCN-EMP-MIC;4:PDG-DOP-DCN-EMP-MIC;5:;6:;
C1 IFREMER, EMP, Microbiol Lab, F-29280 Plouzane, France.
   Cap Atlant, F-44500 La Baule, France.
C2 IFREMER, FRANCE
   CAP ATLANT, FRANCE
SI BREST
SE PDG-DOP-DCN-EMP-MIC
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 1.056
TC 19
UR https://archimer.ifremer.fr/doc/00003/11384/10304.pdf
LA English
DT Article
DE ;Bacteroidales;F-specific RNA bacteriophages;faecal bacterial indicators;microbial source tracking;water
AB Faecal contamination sources were identified in coastal areas around the Guerande-Atlantique peninsula using two microbial source tracking (MST) methods: (i) Bacteroidales host-specific 16S rRNA gene markers measured by real-time PCR and (ii) F-specific bacteriophage (FRNAPH) genotyping. Both methods were used on 63 water samples from 7 water courses. HF183 marker and bacteriophage genogroup II (FRNAPH II) were detected in all water samples and in the majority of water samples, respectively, from La Torre stream (W5), Piriac (W2), R2000 (W3) and Mazy (W7) rain water drains, and also detected, less frequently, in Le Nau drain (W4), suggesting contamination by human faecal sources at these sites. These human markers were weakly detected in Pouliguen channel (W6). Furthermore, BacR and bacteriophage genogroup I (FRNAPH I) were also detected, but at lower concentration and frequency. So, site W6 seems to be contaminated by multiple sources, though mainly human. Finally, BacR was detected twice in Pont d'Armes channel (W1), whereas HF183 was not detected. FRNAPH I and II were detected in only 3 out of 12 water samples. Site W1 seems mainly contaminated by animal sources. As a result of our findings, actions were taken to remediate water and shellfish quality.
PY 2010
SO Water Science And Technology
SN 0273-1223
PU IWA Publishing
VL 61
IS 6
UT 000276181100005
BP 1401
EP 1409
DI 10.2166/wst.2010.033
ID 11384
ER

EF