FN Archimer Export Format PT J TI Stylicins, a new family of antimicrobial peptides from the Pacific blue shrimp Litopenaeus stylirostris BT AF ROLLAND, Jean-Luc ABDELOUAHAB, Mahdia DUPONT, J. LEFEVRE, F. BACHERE, Evelyne ROMESTAND, Bernard AS 1:4;2:2;3:3;4:3;5:4;6:1; FF 1:PDG-DOP-DCM-BOME-LALR;2:;3:;4:;5:PDG-DOP-DCM-BOME;6:; C1 Univ Montpellier 2, IFREMER, CNRS, IRD,UMR Ecosyst Lagunaires 5119, F-34095 Montpellier 5, France. Catholic Univ Louvain, Unite Microbiol MBLA, B-1348 Louvain, Belgium. LibraGen, F-31400 Toulouse, France. IFREMER, Univ Montpellier 2, CNRS, IRD,UMR Ecosyst Lagunaires 5119, F-34095 Montpellier 5, France. C2 UNIV MONTPELLIER, FRANCE UCL, BELGIUM LIBRAGEN, FRANCE IFREMER, FRANCE SI SMEL SETE PALAVAS MONTPELLIER SE PDG-DOP-DCM-BOME-LALR PDG-DOP-DCM-BOME IN WOS Ifremer jusqu'en 2018 copubli-france copubli-europe copubli-univ-france IF 2.916 TC 48 UR https://archimer.ifremer.fr/doc/00003/11387/8094.pdf LA English DT Article DE ;Ls-Stylicin 1;Peptide;Shrimp;Antifungal;Agglutination;Vibrio AB The present study reports the characterization of Ls-Stylicin1, a novel antimicrobial peptide from the penaeid shrimp, Litopenoeus stylirostris. The predicted mature peptide of 82 residues is negatively charged (theoretical pl=5.0) and characterized by a proline-rich N-terminal region and a C-terminal region containing 13 cysteine residues. The recombinant Ls-Stylicin1 has been isolated in both monomeric and dimeric forms. Both display strong antifungal activity against Fusarium oxysporum (1.25 mu M < MIC <2.5 mu M), a pathogenic fungus of shrimp, but lower antimicrobial activity against Gram () bacteria, Vibrio sp. (40 mu M < MIC <80 mu M). However, rLs-Stylicin1 is able to agglutinate Vibrio pennaeicidae in vitro in agreement with its potent LPS-binding activity on immobilized LPS of V. penaeicidae (dissociation constant (K-d) of 9.6 x 10(-8) M). This molecule with no evident homology to other hitherto described antimicrobial peptides but identified herein several species of penaeid shrimp is thought to be the first member of a shrimp antimicrobial peptide family, which we termed stylicins. (C) 2009 Elsevier Ltd. All rights reserved. PY 2010 PD MAR SO Molecular Immunology SN 0161-5890 PU Pergamon-elsevier Science Ltd VL 47 IS 6 UT 000276300600012 BP 1269 EP 1277 DI 10.1016/j.molimm.2009.12.007 ID 11387 ER EF