FN Archimer Export Format
PT J
TI Sperm motility in European hake, Merluccius merluccius, and characterization of its spermatozoa concentration and volume, spermatocrit, osmolality and pH
BT 
AF GROISON, Anne-Laure
   SUQUET, Marc
   COSSON, Jacky
   MAYER, Ian
   SEVERE, Armelle
   BOUQUET, Jean-Marie
   GEFFEN, Audrey J.
   UTNE-PALM, Anne Christine
   KJESBU, Olav Sigurd
AS 1:1,2;2:3;3:4;4:1,6;5:5;6:7;7:1;8:1;9:2;
FF 1:;2:PDG-DOP-DCB-PFOM-PI;3:;4:;5:PDG-DOP-DCB-PFOM-ARN;6:;7:;8:;9:;
C1 Univ Bergen, Dept Biol, N-5020 Bergen, Norway.
   Inst Marine Res, N-5817 Bergen, Norway.
   IFREMER, PFOM ARN, F-29840 Argenton, France.
   Univ P&M Curie Marine Stn, CNRS, UMR 7009, F-06230 Villefranche Sur Mer, France.
   IFREMER, PFOM ARN, F-29280 Plouzane, France.
   Norwegian Sch Vet Sci, N-0033 Oslo, Norway.
   Sars Int Ctr Marine Mol Biol, N-5008 Bergen, Norway.
C2 UNIV BERGEN, NORWAY
   IMR, NORWAY
   IFREMER, FRANCE
   UNIV PARIS 06, FRANCE
   IFREMER, FRANCE
   NVH, NORWAY
   SARS, NORWAY
SI ARGENTON
   BREST
SE PDG-DOP-DCB-PFOM-PI
   PDG-DOP-DCB-PFOM-ARN
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 2.044
TC 11
UR https://archimer.ifremer.fr/doc/00003/11393/8021.pdf
LA English
DT Article
DE ;Cryopreservation;Merluccius merluccius;Osmolality;pH;Sperm motility;Spermatocrit
AB Due to stock declines there is a growing interest in farming of European hake Merluccius merluccius. Thus, knowledge of its sperm biology is of importance not only for purposes of broodstock management, but also for the development of sperm preservation techniques. Hake sperm were collected from mature males caught during the summer-early autumn waters off western Norway and during the winter-early spring in the Bay of Biscay (France). Sperm quality characteristics were assessed after storage at 4 degrees C for 25 +/- 14 h. Average (+/- SD) values for Norwegian and French samples respectively were (i) sperm volume: 3.9 +/- 4.0 and 2.6 +/- 4.0 ml; (ii) spermatozoa concentration: 6.6 +/- 3.2 and 13.9 +/- 5.1 x 10(9) spermatozoa/ml; (iii) spermatocrit: 80.2 +/- 3.3 and 81.8 +/- 10.7%; and (iv) total number of spermatozoa: 23.5 +/- 30.0 and 35.1 +/- 36.2 x 10(9). Average osmolality and pH (+/- SD) of French samples were 349 +/- 28 mOsmol/kg and 7.6 +/- 0.1, respectively. Activation by transfer into full sea water (100 SW) or 10% ovarian fluid in sea water (10 OF) occurred synchronously for virtually all spermatozoa and the percent sperm motile decreased with post activation time. When transferred into 50% sea water diluted with distilled water (50 SW) only a few spermatozoa were activated initially but subsequently reached a maximum percentage of motility followed by a decline. Hake sperm motility declined rapidly to 50% of motility 70 s after activation with 100 SW. Sperm were motile for longer when activated with 50 SW (1570 +/- 295 s) or 10 OF (718 +/- 71 s) compared to 100 SW (317 +/- 121 s). Undiluted hake sperm stored at 4 degrees C up to 10 days retained 10% motility when activated with 100 SW. When cryopreserved, the motility recovery index of the cells at thawing ranged from 0 to 76.4%. These results describe for the first time the sperm traits of European hake following successful cryopreservation, and also show the importance of activation medium on sperm motility. (C) 2010 Elsevier B.V. All rights reserved.
PY 2010
PD MAR
SO Aquaculture
SN 0044-8486
PU Elsevier Science Bv
VL 301
IS 1-4
UT 000276086900005
BP 31
EP 36
DI 10.1016/j.aquaculture.2010.01.026
ID 11393
ER

EF