FN Archimer Export Format
PT J
TI Effect of probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress of Litopenaeus stylirostris under Vibrio nigripulchritudo challenge
BT 
AF CASTEX, Mathieu
   LEMAIRE, Pierrette
   WABETE, Nelly
   CHIM, Liet
AS 1:1;2:1;3:1;4:1;
FF 1:PDG-DOP-DCB-PFOM-PI;2:PDG-DOP-DCOP-LEADNC-LAC;3:PDG-DOP-DCOP-LEADNC-LAC;4:PDG-DOP-DCOP-LEADNC-LAC;
C1 IFREMER, Dept Aquaculture Nouvelle Caledonie, Noumea 9846, New Caledonia.
C2 IFREMER, FRANCE
SI BREST
   SAINT VINCENT
SE PDG-DOP-DCB-PFOM-PI
   PDG-DOP-DCOP-LEADNC-LAC
IN WOS Ifremer jusqu'en 2018
IF 3.044
TC 140
UR https://archimer.ifremer.fr/doc/00003/11419/7984.pdf
LA English
DT Article
DE ;Probiotic;Pediococcus acidilactici;Shrimp;Oxidative stress;Antioxidant status;Vibrio;Immersion challenge
AB Antioxidant defences and induced oxidative stress tissue damage of the blue shrimp Litopenaeus stylirostris, under challenge with Vibrio nigriputchritudo, were investigated for a 72-h period. For this purpose, L stylirostris were first infected by immersion with pathogenic V. nigripulchritudo strain SFn1 and then antioxidant defences: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx), Total antioxidant status (TAS), glutathiones and induced tissue damage (MDA and carbonyl proteins) were determined in the digestive gland at 0, 12, 24, 48 and 72 h post-infection (h.p.i.). In the meantime, TAS was also measured in the blood. Infection level of the shrimps during the challenge was followed by determining V. nigripulchritudo prevalence and load in the haemolymph of the shrimps. Changes in all these parameters during the 72-h.p.i. period were recorded for control shrimps and shrimps previously fed for one month with probiotic Pediococcus aciditactici MA18/5M at 10(7) CFU g(-1) of feed. Our results showed that immersion with V nigripulchritudo led to maximal infection level in the haemolymph at 24 h.p.i. preceding the mortality peak recorded at 48 h.p.i. Significant decreases in the antioxidant defences were detected from 24 h.p.i. and beyond that time infection leaded to increases in oxidative stress level and tissue damage.. Compared to control group, shrimps fed the probiotic diet showed lower infection (20% instead of 45% at 24 h.p.i. in the control group) and mortality (25% instead of 41.7% in the control group) levels. Moreover, infected shrimp fed the probiotic compared to uninfected control shrimps exhibited very similar antioxidant status and oxidative stress level. Compared to the infected control group, shrimps fed the probiotic sustained higher antioxidant defences and lower oxidative stress level. This study shows that bacterial infection leads to oxidative stress in L. stylirostris and highlighted a beneficial effect of P. acidilactici, suggesting both a competitive exclusion effect leading to a reduction of the infection level and/or an enhancement of the antioxidant status of the shrimps. (C) 2010 Elsevier Ltd. All rights reserved.
PY 2010
PD APR
SO Fish & Shellfish Immunology
SN 1050-4648
PU Academic Press Ltd- Elsevier Science Ltd
VL 28
IS 4
UT 000276935700015
BP 622
EP 631
DI 10.1016/j.fsi.2009.12.024
ID 11419
ER

EF