FN Archimer Export Format PT J TI Antibiofilm Activity of the Marine Bacterium Pseudoalteromonas sp Strain 3J6 BT AF DHEILLY, Alexandra SOUM-SOUTERA, Emmanuelle KLEIN, Geraldine BAZIRE, Alexis COMPERE, Chantal HARAS, Dominique DUFOUR, Aurelie AS 1:1;2:1;3:1,2;4:1;5:2;6:1;7:1; FF 1:;2:;3:PDG-DOP-DCB-RDT-IC;4:;5:PDG-DOP-DCB-RDT-IC;6:;7:; C1 Univ Bretagne Sud, LBCM, UEB, EA 3884, F-56321 Lorient, France. IFREMER, Serv Interface & Capteurs, Plouzane, France. C2 UBS, FRANCE IFREMER, FRANCE SI BREST SE PDG-DOP-DCB-RDT-IC IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 3.778 TC 67 UR https://archimer.ifremer.fr/doc/00004/11530/11317.pdf LA English DT Article AB Biofilm formation results in medical threats or economic losses and is therefore a major concern in a variety of domains. In two-species biofilms of marine bacteria grown under dynamic conditions, Pseudoalteromonas sp. strain 3J6 formed mixed biofilms with Bacillus sp. strain 4J6 but was largely predominant over Paracoccus sp. strain 4M6 and Vibrio sp. strain D01. The supernatant of Pseudoalteromonas sp. 3J6 liquid culture (SN3J6) was devoid of antibacterial activity against free-living Paracoccus sp. 4M6 and Vibrio sp. D01 cells, but it impaired their ability to grow as single-species biofilms and led to higher percentages of nonviable cells in 48-h biofilms. Antibiofilm molecules of SN3J6 were able to coat the glass surfaces used to grow biofilms and reduced bacterial attachment about 2-fold, which might partly explain the biofilm formation defect but not the loss of cell viability. SN3J6 had a wide spectrum of activity since it affected all Gram-negative marine strains tested except other Pseudoalteromonas strains. Biofilm biovolumes of the sensitive strains were reduced 3-to 530-fold, and the percentages of nonviable cells were increased 3-to 225-fold. Interestingly, SN3J6 also impaired biofilm formation by three strains belonging to the human-pathogenic species Pseudomonas aeruginosa, Salmonella enterica, and Escherichia coli. Such an antibiofilm activity is original and opens up a variety of applications for Pseudoalteromonas sp. 3J6 and/or its active exoproducts in biofilm prevention strategies. PY 2010 PD JUL SO Applied And Environmental Microbiology SN 0099-2240 PU Amer Soc Microbiology VL 76 IS 11 UT 000277871400006 BP 3452 EP 3461 DI 10.1128/AEM.02632-09 ID 11530 ER EF