FN Archimer Export Format PT J TI Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality BT AF FLEURY, Elodie MOAL, Jeanne BOULO, Viviane DANIEL, Jean-Yves MAZURAIS, David HENAUT, Alain CORPOREAU, Charlotte BOUDRY, Pierre FAVREL, Pascal HUVET, Arnaud AS 1:1;2:1;3:2;4:1;5:;6:;7:1;8:1;9:1;10:1; FF 1:PDG-DOP-DCB-PFOM-PI;2:PDG-DOP-DCB-PFOM-PI;3:PDG-DOP-DCM-BOME-LALR;4:PDG-DOP-DCB-PFOM-PI;5:PDG-DOP-DCB-PFOM-ARN;6:;7:PDG-DOP-DCB-PFOM-PI;8:PDG-DOP-DCB-PFOM-PI;9:;10:PDG-DOP-DCB-PFOM-PI; C1 IFREMER, UMR Nutr Aquaculture & Genom 1067, Ctr Brest, F-29280 Plouzane, France. Univ Paris 06, CNRS, UMR Systemat 7138, F-75252 Paris, France. C2 IFREMER, FRANCE UNIV PARIS 06, FRANCE SI BREST MONTPELLIER SE PDG-DOP-DCB-PFOM-PI PDG-DOP-DCM-BOME-LALR PDG-DOP-DCB-PFOM-ARN IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.962 TC 46 UR https://archimer.ifremer.fr/doc/00005/11590/8258.pdf LA English DT Article DE ;Mollusca;Bivalvia;Gene expression;cDNA microarrays;Reproduction;Oxidative stress;Energy metabolism AB Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. Heredity appears to be a major factor determining the sensitivity of oysters to summer mortality, allowing resistant (R) and susceptible (S) lines to be produced. We conducted genome-wide expression profiling of R and S gonads during the 3-month period preceding a summer mortality event, using a cDNA microarray that we designed. ANOVA analysis revealed that 34 genes were differentially expressed between R and S lines on four dates preceding the mortality event. Annotation of some of these genes highlights reproduction and its allocation and antioxidant defenses as the main pathways that operate differentially between R and S lines. This transcriptional analysis provides new indications to define markers for quantitative trait loci searches and functional studies and evaluate the potential role of each gene in the resistance to summer mortality. PY 2010 PD JUL SO Marine Biotechnology SN 1436-2228 PU Springer VL 12 IS 3 UT 000278095400011 BP 326 EP 339 DI 10.1007/s10126-009-9227-9 ID 11590 ER EF