FN Archimer Export Format PT J TI Vagococcus penaei sp. nov., isolated from spoilage microbiota of cooked shrimp (Penaeus vannamei) BT AF JAFFRES, Emmanuel PREVOST, Herve ROSSERO, Albert JOFFRAUD, Jean-Jacques DOUSSET, Xavier AS 1:1,2;2:1;3:1;4:2;5:1; FF 1:;2:;3:;4:PDG-DOP-DCN-BRM-STBM;5:; C1 UMR INRA 1014 SECALIM ENITIAA, F-44322 Nantes 3, France. IFREMER, Lab Sci & Technol Biomasse Marine, F-44311 Nantes, France. C2 INRA, FRANCE IFREMER, FRANCE SI NANTES SE PDG-DOP-DCN-BRM-STBM IN WOS Ifremer jusqu'en 2018 copubli-france copubli-p187 IF 1.93 TC 28 UR https://archimer.ifremer.fr/doc/00006/11709/8365.pdf LA English DT Article AB A polyphasic taxonomic study, using phenotypic and phylogenetic characterization as well as genotypic methods, was performed on five unknown Gram-positive, catalase-negative, coccus-shaped Vagococcus-like bacteria isolated from the spoilage microbiota of cooked shrimp. Comparative 16S rRNA gene sequence analysis indicated that these strains belonged to the genus Vagococcus and that it formed a new branch distinct from the six species already recognized (V. fluvialis, V. salmoninarum, V. lutrae, V. fessus, V. carniphilus and V. elongatus). The polyphasic approach on the representative strain CD276T, including DNA–DNA hybridizations, pulsed-field gel electrophoresis of whole-genome DNA profiles, G+C content determination, cell-wall peptidoglycan typing, fatty acid analysis and extensive biochemical characterization, was used to clarify the taxonomic position of this strain. These data confirmed that CD276T (LMG 24833T = CIP 109914T) represents the type strain of a novel species, for which the name Vagococcus penaei sp. nov. is proposed. PY 2010 PD SEP SO International Journal Of Systematic And Evolutionary Microbiology SN 1466-5026 PU Soc General Microbiology VL 60 UT 000282543600030 BP 2159 EP 2164 DI 10.1099/ijs.0.012872-0 ID 11709 ER EF