FN Archimer Export Format
PT J
TI Evaluation of various real-time RT-PCR assays for the detection and quantitation of human norovirus
BT 
AF BUTOT, Sophie
   LE GUYADER, Soizick
   KROL, Joanna
   PUTALLAZ, Thierry
   AMOROSO, Richard
   SANCHEZ, Gloria
AS 1:1;2:2;3:2;4:1;5:1;6:1;
FF 1:;2:PDG-DOP-DCN-EMP-MIC;3:PDG-DOP-DCN-EMP-MIC;4:;5:;6:;
C1 Nestle Res Ctr, Qual & Safety Assurance Dept, CH-1000 Lausanne, Switzerland.
   IFREMER, Microbiol Lab, F-44311 Nantes 03, France.
C2 NESTLE RES CTR, SWITZERLAND
   IFREMER, FRANCE
SI NANTES
SE PDG-DOP-DCN-EMP-MIC
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
IF 2.139
TC 39
UR https://archimer.ifremer.fr/doc/00006/11760/8473.pdf
LA English
DT Article
DE ;Norovirus;Real-time RT-PCR;Commercial kit
AB Human noroviruses (NoVs) are the most common viruses causing acute gastroenteritis in humans. Performance characteristics of two commercial quantitative NoV RT-PCR assays, the Norovirus real-time RT-PCR Kit (AnDiaTec) and the Type I and Type II kits (Generon), and the international assay as selected by the CEN/TC/WG6/TAG4 group were evaluated for the specific detection and quantitation of 59 NoV samples, including different subtypes of NoV genogroup I and II. The results showed that the method proposed by the CEN/TC/WG6/TAG4 group was 100% specific since it was able to detect all samples tested. The commercialized kits evaluated failed to detect a vast majority of NoV GI strains. Additionally the Generon kit did not succeed to detect strains from GII.3, GII.5, GII.6, GII.7, GII.8, GII.12 and GII.17. In addition, the detection limit using the most prevalent strain, NoV GII.4, was 2.5 PCRU per reaction using both commercial kits. Despite this good sensitivity for NoV GII.4 detection it is concluded that both commercial assays are not suitable for the detection and quantitation of most NOV subtypes. Therefore the method proposed by the CEN/TC/WG6/TAG4 group is recommended for epidemiological studies and outbreaks investigations. (C) 2010 Elsevier B.V. All rights reserved.
PY 2010
PD JUN
SO Journal Of Virological Methods
SN 0166-0934
PU Elsevier Science Bv
VL 167
IS 1
UT 000278782700015
BP 90
EP 94
DI 10.1016/j.jviromet.2010.03.018
ID 11760
ER

EF