FN Archimer Export Format PT J TI Requirements for screening and confirmatory methods for the detection and quantification of marine biotoxins in end-product and official control BT AF HESS, Philipp AS 1:; FF 1:PDG-ODE-LITTORAL-PHYC; C1 IFREMER, Ctr Nantes, Dept Environm Microbiol & Phycotoxines, F-44311 Nantes 03, France. C2 IFREMER, FRANCE SI NANTES SE PDG-ODE-LITTORAL-PHYC IN WOS Ifremer jusqu'en 2018 IF 3.841 TC 32 UR https://archimer.ifremer.fr/doc/00006/11767/8989.pdf LA English DT Article DE ;Bioanalytical methods;Bioassays;Mass spectrometry;Liquid chromatography;Biological samples AB An overview is given of the biological origin of phycotoxins, as well as their chemical characteristics. Major poisoning types are described and examples of poisoning events are given to illustrate the importance of the phenomenon to both shellfish consumers and the shellfish producing industry. The characteristics of phycotoxins as natural products, the lack of predictability of their occurrence, economic drivers and the freshness of shellfish consumed in many countries result in a number of requirements for methods to be used in the efficient detection of these compounds. Subsequently, the performance of mouse bioassays and mass spectrometry as detection tools are compared for examples from Irish and French monitoring programmes to assess the usefulness of qualitative and quantitative tools in official control, and their fitness for purpose compared with the requirements. The final part of the paper critically reviews methods available for the end-product and official control of shellfish toxins and their use in screening and confirmatory approaches in monitoring. Recent expert consultations on the methodology for phycotoxins at European and global level are summarised and recommendations are made for future progress in this area. PY 2010 PD JUN SO Analytical And Bioanalytical Chemistry SN 1618-2642 PU Springer Heidelberg VL 397 IS 5 UT 000278810000009 BP 1683 EP 1694 DI 10.1007/s00216-009-3444-y ID 11767 ER EF