FN Archimer Export Format
PT J
TI Differential protein expression profile in the liver of pikeperch (Sander lucioperca) larvae fed with increasing levels of phospholipids
BT 
AF HAMZA, Neila
   SILVESTRE, Frederic
   MHETLI, Mohamed
   BEN KHEMIS, Ines
   DIEU, Marc
   RAES, Martine
   CAHU, Chantal
   KESTEMONT, Patrick
AS 1:1,2;2:2;3:1;4:1;5:3;6:3;7:4;8:2;
FF 1:;2:;3:;4:;5:;6:;7:PDG-DOP-DCB-PFOM;8:;
C1 Inst Natl Sci & Technol Mer, Salammbo 2025, Tunisia.
   Fac Univ Notre Dame Paix, URBO, B-5000 Namur, Belgium.
   Fac Univ Notre Dame Paix, URBC, B-5000 Namur, Belgium.
   IFREMER, UMR 1067, F-29280 Plouzane, France.
C2 INSTM, TUNISIA
   UNIV UNAMUR, BELGIUM
   UNIV UNAMUR, BELGIUM
   IFREMER, FRANCE
SI BREST
SE PDG-DOP-DCB-PFOM
IN WOS Ifremer jusqu'en 2018
   copubli-europe
   copubli-int-hors-europe
   copubli-sud
IF 1.8
TC 14
UR https://archimer.ifremer.fr/doc/00007/11837/8575.pdf
LA English
DT Article
DE ;2D-DIGE;Metabolism;Phospholipid;Pikeperch;Proteomics
AB A comparative proteomic approach was used to assess the protein expression profile in the liver of 34 days old pikeperch larvae fed from day 10 post hatching, with three isoproteic and isolipidic formulated diets varying by their phospholipid (PL) contents (% dry diet weight): 1.4% (PL1), 4.7% (PL5) and 9.5% (PL9). Using 2D-DIGE minimal labelling of liver extracts, we were able to show 56 protein spots with a differential intensity (p<0.05) depending on the dietary PL content. Among these spots, 11 proteins were un-ambiguously identified using nanoLC-MS/MS tandem mass spectrometry. In the PL9 larvae, our results indicate that the glycolytic pathway could be down-regulated due to the under-expression of the fructose biphosphate aldolase B and the phosphoglucomutase 1. Meanwhile, propionyl coenzyme A carboxylase (a gluconeogenic enzyme) was under-expressed. In addition, another gluconeogenic and lipogenic enzyme, pyruvate carboxylase, was identified in 3 different spots as being under-expressed in fish fed with the intermediate PL level (PL5). A high PL content increased the expression of sarcosine dehydrogenase, an enzyme involved in methionine metabolism, along with vinculin, a structural protein. Moreover, several stress proteins (glutathione S-transferase M, glucose regulated protein 75 and peroxiredoxin-1) were modulated in response to the dietary PL level and fatty acid composition. In the larvae fed with the lowest dietary PL content (PL1), over-expression of both GSTM and GRP75 might indicate a cellular stress in this experimental treatment, while the under-expression of Prx1 might indicate a lower defence against oxidative stress. In conclusion, this nutriproteomic approach showed significant modifications of protein expression in the liver of pikeperch larvae fed different PL contents, highlighting the importance of these nutrients and their influence on metabolism processes and on stress response. (C) 2010 Elsevier Inc. All rights reserved.
PY 2010
PD JUL
SO Comparative Biochemistry And Physiology D-genomics & Proteomics
SN 1744-117X
PU Elsevier Science Inc
VL 5
IS 2
UT 000279214000007
BP 130
EP 137
DI 10.1016/j.cbd.2010.03.005
ID 11837
ER

EF