Regulation of FADS2 expression and activity in European sea bass (Dicentrarchus labrax, L.) fed a vegetable diet

Type Article
Date 2010-08
Language English
Author(s) Geay Florian1, Culi E. Santigosa I.1, Corporeau Charlotte2, Boudry PierreORCID2, Dreano Y.3, 4, Corcos L.3, 4, Bodin Nathalie5, Vandeputte Marc6, 7, Zambonino-Infante Jose-LuisORCID1, Mazurais DavidORCID1, Cahu Chantal1
Affiliation(s) 1 : IFREMER, Ifremer Marine Fish Nutr Team, UMR 1067, Nutr Aquaculture & Genom Res Unit, F-29280 Plouzane, France.
2 : Univ Caen, UMR 100, PE2M, IFREMER, Plouzane, France.
3 : Univ Brest, INSERM, U613, ECLA, F-29200 Brest, France.
4 : Univ Europeenne Bretagne, Fac Med, F-29200 Brest, France.
5 : Inst Rech & Dev, CRH, EME, UMR 212, F-34203 Sete, France.
6 : IFREMER, F-34250 Palavas Les Flats, France.
7 : INRA, UMR 1313, F-78350 Jouy En Josas, France.
Source Comparative Biochemistry And Physiology B-biochemistry & Molecular Biology (1096-4959) (Elsevier Science Inc), 2010-08 , Vol. 156 , N. 4 , P. 237-243
DOI 10.1016/j.cbpb.2010.03.008
WOS© Times Cited 46
Keyword(s) Sea bass (Dicentrarchus labrax), FADS2 expression and activity, HUFA biosynthesis
Abstract Supplies of marine fish oils are limited, and continued growth in aquaculture production dictates that lipid substitutes in fish diets must be used without compromising fish health and product quality. In this study, the total substitution of a fish meal and fish oil by a blend of vegetable meals (corn, soybean, wheat and lupin) and linseed oil in the diet of European sea bass (Dicentrachus labrax) was investigated. Two groups of European sea bass were fed with fish diet (FD) or vegetable diet (VD) for 9 months. VD, totally deprived of eicosapentaenoate (EPA; 20:5n-3) and docosahexaenoate (DHA; 22:6n-3), revealed a nutritional deficiency and affected growth performance. Whilst VD induced a significant increase in fatty acid desaturase 2 (FADS2) and sterol binding regulatory element-binding protein 1 (SREBP-1) mRNA levels, the desaturation rate of [1-C-14]18:3n-3 into [1-C-14]18:4n-3, analysed in microsomal preparations using HPLC method, did not show an upregulation of FADS2 activities in liver and intestine of fish fed VD. Moreover Western-blot analysis did not revealed any significant difference of FADS2 protein amount between the two dietary groups. These data demonstrate that sea bass exhibits a desaturase (FADS2) activity whatever their diet, but a post-transcriptional regulation of fads2 RNA prevents an increase of enzyme in fish fed a HUFA-free diet. This led to a lower fish growth and poor muscle HUFA content. (C) 2010 Elsevier Inc. All rights reserved.
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Geay Florian, Culi E. Santigosa I., Corporeau Charlotte, Boudry Pierre, Dreano Y., Corcos L., Bodin Nathalie, Vandeputte Marc, Zambonino-Infante Jose-Luis, Mazurais David, Cahu Chantal (2010). Regulation of FADS2 expression and activity in European sea bass (Dicentrarchus labrax, L.) fed a vegetable diet. Comparative Biochemistry And Physiology B-biochemistry & Molecular Biology, 156(4), 237-243. Publisher's official version : https://doi.org/10.1016/j.cbpb.2010.03.008 , Open Access version : https://archimer.ifremer.fr/doc/00008/11929/