FN Archimer Export Format
PT J
TI Regulation of FADS2 expression and activity in European sea bass (Dicentrarchus labrax, L.) fed a vegetable diet
BT 
AF GEAY, Florian
   CULI, E. Santigosa I.
   CORPOREAU, Charlotte
   BOUDRY, Pierre
   DREANO, Y.
   CORCOS, L.
   BODIN, Nathalie
   VANDEPUTTE, Marc
   ZAMBONINO-INFANTE, Jose-Luis
   MAZURAIS, David
   CAHU, Chantal
AS 1:1;2:1;3:2;4:2;5:3,4;6:3,4;7:5;8:6,7;9:1;10:1;11:1;
FF 1:PDG-DOP-DCB-PFOM-ARN;2:;3:PDG-DOP-DCB-PFOM-PI;4:PDG-DOP-DCB-PFOM-PI;5:;6:;7:;8:;9:PDG-DOP-DCB-PFOM-ARN;10:PDG-DOP-DCB-PFOM-ARN;11:PDG-DOP-DCB-PFOM;
C1 IFREMER, Ifremer Marine Fish Nutr Team, UMR 1067, Nutr Aquaculture & Genom Res Unit, F-29280 Plouzane, France.
   Univ Caen, UMR 100, PE2M, IFREMER, Plouzane, France.
   Univ Brest, INSERM, U613, ECLA, F-29200 Brest, France.
   Univ Europeenne Bretagne, Fac Med, F-29200 Brest, France.
   Inst Rech & Dev, CRH, EME, UMR 212, F-34203 Sete, France.
   IFREMER, F-34250 Palavas Les Flats, France.
   INRA, UMR 1313, F-78350 Jouy En Josas, France.
C2 IFREMER, FRANCE
   UNIV CAEN, FRANCE
   UBO, FRANCE
   UEB, FRANCE
   IRD, FRANCE
   IFREMER, FRANCE
   INRA, FRANCE
SI BREST
   SETE
   PALAVAS
SE PDG-DOP-DCB-PFOM-ARN
   PDG-DOP-DCB-PFOM-PI
   IRD
   PDG-DOP-DCM-BOME-LALR
   PDG-DOP-DCB-PFOM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-univ-france
IF 1.989
TC 56
UR https://archimer.ifremer.fr/doc/00008/11929/8635.pdf
LA English
DT Article
DE ;Sea bass (Dicentrarchus labrax);FADS2 expression and activity;HUFA biosynthesis
AB Supplies of marine fish oils are limited, and continued growth in aquaculture production dictates that lipid substitutes in fish diets must be used without compromising fish health and product quality. In this study, the total substitution of a fish meal and fish oil by a blend of vegetable meals (corn, soybean, wheat and lupin) and linseed oil in the diet of European sea bass (Dicentrachus labrax) was investigated. Two groups of European sea bass were fed with fish diet (FD) or vegetable diet (VD) for 9 months. VD, totally deprived of eicosapentaenoate (EPA; 20:5n-3) and docosahexaenoate (DHA; 22:6n-3), revealed a nutritional deficiency and affected growth performance. Whilst VD induced a significant increase in fatty acid desaturase 2 (FADS2) and sterol binding regulatory element-binding protein 1 (SREBP-1) mRNA levels, the desaturation rate of [1-C-14]18:3n-3 into [1-C-14]18:4n-3, analysed in microsomal preparations using HPLC method, did not show an upregulation of FADS2 activities in liver and intestine of fish fed VD. Moreover Western-blot analysis did not revealed any significant difference of FADS2 protein amount between the two dietary groups. These data demonstrate that sea bass exhibits a desaturase (FADS2) activity whatever their diet, but a post-transcriptional regulation of fads2 RNA prevents an increase of enzyme in fish fed a HUFA-free diet. This led to a lower fish growth and poor muscle HUFA content. (C) 2010 Elsevier Inc. All rights reserved.
PY 2010
PD AUG
SO Comparative Biochemistry And Physiology B-biochemistry & Molecular Biology
SN 1096-4959
PU Elsevier Science Inc
VL 156
IS 4
UT 000279298400001
BP 237
EP 243
DI 10.1016/j.cbpb.2010.03.008
ID 11929
ER

EF