FN Archimer Export Format
PT J
TI Impact of ultrafiltration and nanofiltration of an industrial fish protein hydrolysate on its bioactive properties
BT 
AF PICOT, Laurent
   RAVALLEC, Rozenn
   FOUCHEREAU-PERON, Martine
   VANDANJON, Laurent
   JAOUEN, Pascal
   CHAPLAIN-DEROUINIOT, Maryse
   GUERARD, Fabienne
   CHABEAUD, Aurelie
   LEGAL, Yves
   MARTINEZ ALVAREZ, Oscar
   BERGE, Jean-Pascal
   PIOT, Jean-Marie
   BATISTA, Irineu
   PIRES, Carla
   THORKELSSON, Gudjon
   DELANNOY, Charles
   JAKOBSEN, Greta
   JOHANSSON, Inger
   BOURSEAU, Patrick
AS 1:1;2:2;3:3;4:4,5;5:4;6:4;7:6;8:6;9:3;10:3,7;11:8;12:1;13:11;14:11;15:9,10;16:;17:;18:;19:4,5;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:PDG-DOP-DCN-BRM-STBM;12:;13:;14:;15:;16:;17:;18:PDG-DOP-DCN-BE-LBCO;19:;
C1 Univ Rochelle, CNRS, UMR 6250, LIENSs, La Rochelle, France.
   IUT A Polytech Lille, UPRES EA 1026, ProBioGEM, Lille, France.
   UPMC, UMR BOREA, MNHN 7208, CNRS,Stn Biol Marine,IRD 207, Concarneau, France.
   Univ Nantes, CNRS, UMR 6144, GEPEA, St Nazaire, France.
   Univ Bretagne Sud, LIMATB, Lorient, France.
   Univ Bretagne Occidentale, ANTiOX, Quimper, France.
   CSIC, Inst Frio, E-28040 Madrid, Spain.
   STAM, IFREMER, Nantes, France.
   Maris Ohf, Reykjavik, Iceland.
   Univ Iceland, Reykjavik, Iceland.
   Ipimar, Lisbon, Portugal.
C2 UNIV LA ROCHELLE, FRANCE
   UNIV LILLE, FRANCE
   UNIV PARIS 06, FRANCE
   UNIV NANTES, FRANCE
   UBS, FRANCE
   UBO, FRANCE
   CSIC, SPAIN
   IFREMER, FRANCE
   MARIS OHF, ICELAND
   UNIV ICELAND, ICELAND
   IPIMAR, PORTUGAL
SI NANTES
SE PDG-DOP-DCN-BRM-STBM
   PDG-DOP-DCN-BE-LBCO
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
   copubli-int-hors-europe
IF 1.36
TC 91
UR https://archimer.ifremer.fr/doc/00011/12217/9259.pdf
LA English
DT Article
DE ;fish protein hydrolysate;ultrafiltration;nanofiltration;membrane separation;fractionation process;bioactive peptide
AB BACKGROUND: Numerous studies have demonstrated that in vitro controlled enzymatic hydrolysis of fish and shellfish proteins leads to bioactive peptides. Ultrafiltration (UF) and/or nanofiltration (NF) can be used to refine hydrolysates and also to fractionate them in order to obtain a peptide population enriched in selected sizes. This study was designed to highlight the impact of controlled UF and NF on the stability of biological activities of an industrial fish protein hydrolysate (FPH) and to understand whether fractionation could improve its content in bioactive peptides. RESULTS: The starting fish protein hydrolysate exhibited a balanced amino acid composition, a reproducible molecular weight (MW) profile, and a low sodium chloride content, allowing the study of its biological activity. Successive fractionation on UF and NF membranes allowed concentration of peptides of selected sizes, without, however, carrying out sharp separations, some MW classes being found in several fractions. Peptides containing Pro, Hyp, Asp and Glu were concentrated in the UF and NF retentates compared to the unfractionated hydrolysate and UF permeate, respectively. Gastrin/cholecystokinin-like peptides were present in the starting FPH, UF and NF fractions, but fractionation did not increase their concentration. In contrast, quantification of calcitonin gene-related peptide (CGRP)-like peptides demonstrated an increase in CGRP-like activities in the UF permeate, relative to the starting FPH. The starting hydrolysate also showed a potent antioxidant and radical scavenging activity, and a moderate angiotensin-converting enzyme (ACE)-1 inhibitory activity, which were not increased by UF and NF fractionation. CONCLUSION: Fractionation of an FPH using membrane separation, with a molecular weight cut-off adapted to the peptide composition, may provide an effective means to concentrate CGRP-like peptides and peptides enriched in selected amino acids. The peptide size distribution observed after UF and NF fractionation demonstrates that it is misleading to characterize the fractions obtained by membrane filtration according to the MW cut-off of the membrane only, as is currently done in the literature. (C) 2010 Society of Chemical Industry
PY 2010
PD AUG
SO Journal Of The Science Of Food And Agriculture
SN 0022-5142
PU John Wiley & Sons Ltd
VL 90
IS 11
UT 000280482900008
BP 1819
EP 1826
DI 10.1002/jsfa.4020
ID 12217
ER

EF