FN Archimer Export Format PT J TI Detection and description of a particular Ostreid herpesvirus 1 genotype associated with massive mortality outbreaks of Pacific oysters, Crassostrea gigas, in France in 2008 BT AF SEGARRA, Amelie PEPIN, Jean-Francois ARZUL, Isabelle MORGA, Benjamin FAURY, Nicole RENAULT, Tristan AS 1:1;2:1;3:1;4:1;5:1;6:1; FF 1:PDG-DOP-DCN-AGSAE-LGP;2:PDG-DOP-DCN-AGSAE-LGP;3:PDG-DOP-DCN-AGSAE-LGP;4:PDG-DOP-DCN-AGSAE-LGP;5:PDG-DOP-DCN-AGSAE-LGP;6:PDG-DOP-DCN-AGSAE-LGP; C1 Inst Francais Rech Exploitat Mer, Lab Genet & Pathol, F-17390 La Tremblade, France. C2 IFREMER, FRANCE SI LA TREMBLADE SE PDG-DOP-DCN-AGSAE-LGP IN WOS Ifremer jusqu'en 2018 IF 2.905 TC 314 UR https://archimer.ifremer.fr/doc/00011/12260/9035.pdf LA English DT Article DE ;Herpes virus;Oyster;Crassostrea gigas;OsHV-1 mu Var;Increased mortality;Microsatellites AB Ostreid herpesvirus 1 (OsHV-1) infections have been reported around the world and are associated with high mortalities of the Pacific oyster (Crassostrea gigas). In the summer 2008, abnormal mortality rates ranging from 80% to 100% were reported in France and affected only Pacific oysters. Analyses of oyster samples collected during mortality outbreaks demonstrated a significant detection of OsHV-1 (75% of analysed batches), which appeared stronger than previous years. DNA sequencing based on C and IA regions was carried out on 28 batches of OsHV-1 infected Pacific oysters collected in 2008. Polymorphisms were described in both the C and IA regions and characterized a genotype of OsHV-1 not already reported and termed OsHV-1 μVar. A microsatellite zone present in the C region showed several deletions. Additionally, 44 isolates collected in France and in the USA, from 1995 to 2007 were sequenced and compared to the 2008 sequences. The analyses of 76 sequences showed OsHV-1 μVar detection only in 2008 isolates. These data suggest that OsHV-1 μVar can be assumed as an emergent genotype. PY 2010 PD OCT SO Virus Research SN 0168-1702 PU Elsevier Science Bv VL 153 IS 1 UT 000282486100012 BP 92 EP 99 DI 10.1016/j.virusres.2010.07.011 ID 12260 ER EF