TY - JOUR T1 - Development of four EST-SSR multiplex PCRs in the Pacific oyster (Crassostrea gigas) and their validation in parentage assignment A1 - Li,Ronghua A1 - Li,Qi A1 - Cornette,Florence A1 - Degremont,Lionel A1 - Lapegue,Sylvie AD - IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France. AD - Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China. UR - https://doi.org/10.1016/j.aquaculture.2010.09.037 DO - 10.1016/j.aquaculture.2010.09.037 KW - Pacific oyster KW - Crassostrea gigas KW - Microsatellite KW - EST KW - Multiplex PCR KW - Parentage assignment N2 - We report four highly informative multiplex PCRs developed from twelve previously described ESTSSRs in Crassostrea gigas. We evaluated and validated these multiplex PCRs in twelve full-sib families. The average allelic richness and the polymorphism information content (PIC) were 11.1 and 0.811 respectively. The combined power of exclusion was greater than 99.99% using all four multiplex assays. A hundred and forty three tests of segregation ratios revealed 11 significant departures from expected Mendelian ratios. The frequency of null alleles was estimated as 4.9% of all the alleles segregating based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 97% of all offspring were unambiguously allocated to a pair of parents based on two multiplex PCRs with only a 4% error rate, and 100% of the offspring were correctly allocated to their parents when three multiplex PCRs were used. Y1 - 2010/12 PB - Elsevier Science Bv JF - Aquaculture SN - 0044-8486 VL - 310 IS - 1-2 SP - 234 EP - 239 ID - 12419 ER -