FN Archimer Export Format
PT J
TI Development of four EST-SSR multiplex PCRs in the Pacific oyster (Crassostrea gigas) and their validation in parentage assignment
BT 
AF LI, Ronghua
   LI, Qi
   CORNETTE, Florence
   DEGREMONT, Lionel
   LAPEGUE, Sylvie
AS 1:1,2;2:2;3:1;4:1;5:1;
FF 1:;2:;3:PDG-DOP-DCN-AGSAE-LGP;4:PDG-DOP-DCN-AGSAE-LGP;5:PDG-DOP-DCN-AGSAE-LGP;
C1 IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
   Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China.
C2 IFREMER, FRANCE
   UNIV OCEAN CHINA, CHINA
SI BREST
   LA TREMBLADE
SE PDG-DOP-DCB-PFOM-PI
   PDG-DOP-DCN-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
   copubli-sud
IF 2.044
TC 25
UR https://archimer.ifremer.fr/doc/00013/12419/9999.pdf
LA English
DT Article
DE ;Pacific oyster;Crassostrea gigas;Microsatellite;EST;Multiplex PCR;Parentage assignment
AB We report four highly informative multiplex PCRs developed from twelve previously described ESTSSRs in Crassostrea gigas. We evaluated and validated these multiplex PCRs in twelve full-sib families. The average allelic richness and the polymorphism information content (PIC) were 11.1 and 0.811 respectively. The combined power of exclusion was greater than 99.99% using all four multiplex assays. A hundred and forty three tests of segregation ratios revealed 11 significant departures from expected Mendelian ratios. The frequency of null alleles was estimated as 4.9% of all the alleles segregating based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 97% of all offspring were unambiguously allocated to a pair of parents based on two multiplex PCRs with only a 4% error rate, and 100% of the offspring were correctly allocated to their parents when three multiplex PCRs were used.
PY 2010
PD DEC
SO Aquaculture
SN 0044-8486
PU Elsevier Science Bv
VL 310
IS 1-2
UT 000286161200034
BP 234
EP 239
DI 10.1016/j.aquaculture.2010.09.037
ID 12419
ER

EF