FN Archimer Export Format
PT J
TI Sperm features of captive Atlantic bluefin tuna (Thunnus thynnus)
BT 
AF SUQUET, Marc
   COSSON, J.
   DE LA GANDARA, F.
   MYLONAS, C. C.
   PAPADAKI, M.
   LALLEMANT, S.
   FAUVEL, Christian
AS 1:1;2:2;3:3;4:4;5:4;6:5;7:5;
FF 1:PDG-DOP-DCB-PFOM-PI;2:;3:;4:;5:;6:;7:PDG-DOP-DCM-BOME-LALR;
C1 IFREMER, PFOM PI, Stn Expt Argenton, F-29840 Argenton, France.
   Univ Paris 06, CNRS, UMR 7009, Stn Marine, Villefranche Sur Mer, France.
   Ctr Oceanog Murcia, IEO, Murcia, Spain.
   Hellen Ctr Marine Res, Inst Aquaculture, Iraklion, Greece.
   IFREMER, Stn Expt Aquaculture, Palavas Les Flots, France.
C2 IFREMER, FRANCE
   UNIV PARIS 06, FRANCE
   IEO, SPAIN
   HELLEN CTR MARINE RES, GREECE
   IFREMER, FRANCE
SI ARGENTON
   PALAVAS
SE PDG-DOP-DCB-PFOM-PI
   PDG-DOP-DCM-BOME-LALR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 0.945
TC 11
UR https://archimer.ifremer.fr/doc/00015/12645/9722.pdf
LA English
DT Article
AB P>The present study aimed to establish some basic characteristics of Atlantic bluefin tuna sperm from captive mature males, treated or untreated by gonadotropin releasing hormones agonist (GnRHa). Intratesticular milt was collected from treated and untreated fish (mean weight +/- SD: 122.9 +/- 29.2 kg, n = 21). There was no significant effect of GnRHa treatment on GSI (1.33 +/- 0.70%, n = 21) or on sperm concentration (3.8 +/- 1.3 x 1010 spermatozoa ml-1, n = 21) estimated by optical density at 260 nm. Similarly, the percentage of motile spermatozoa measured at 30 s post activation: activating medium (AM seawater containing 10 mg ml-1 BSA) was not significantly different between control and GnRHa implanted males. On the other hand, a significantly higher Average Path Velocity (VAP) was assessed for sperm from GnRHa-treated fish, compared to controls. Regardless of hormonal treatment, the percentage of motile sperm decreased after a plateau of 5-6 min post activation, and any forwardly progressing movement ceased after 10-11 min. Linear trajectories of spermatozoa were observed in seawater while tighter circles were assessed when increasing the Ca2+concentration in the AM. The storage capacity at 4 degrees C was significantly lowered when NAM (Non Activating Medium: 50% seawater containing 10 mg ml-1 BSA) was added to sperm at a 1 : 1 dilution. These results demonstrated that treatment with GnRHa had little effects (except on VAP) on sperm characteristics in captive reared Atlantic bluefin tuna. Further investigations are required to improve the knowledge of tuna sperm biology and, in this respect, the comparison between intratesticular and released sperm features should be highly informative.
PY 2010
PD OCT
SO Journal Of Applied Ichthyology
SN 0175-8659
PU Wiley-blackwell Publishing, Inc
VL 26
IS 5
UT 000281894400026
BP 775
EP 778
DI 10.1111/j.1439-0426.2010.01533.x
ID 12645
ER

EF