FN Archimer Export Format PT J TI Non-specific activation of antiviral immunity and induction of RNA interference may engage the same pathway in the Pacific white leg shrimp Litopenaeus vannamei BT AF LABREUCHE, Yannick VELOSO, Artur DE LA VEGA, Enrique GROSS, Paul S. CHAPMAN, Robert W. BROWDY, Craig L. WARR, Gregory W. AS 1:1,4;2:1;3:1;4:2;5:3;6:3;7:1,2; FF 1:PDG-DOP-DCOP-LEADNC-LAC;2:;3:;4:;5:;6:;7:; C1 Med Univ S Carolina, Marine Biomed & Environm Sci Ctr, Charleston, SC 29412 USA. Med Univ S Carolina, Dept Biochem & Mol Biol, Charleston, SC 29425 USA. Marine Resources Inst, S Carolina Dept Nat Resources, Charleston, SC 29412 USA. IFREMER, Département Lagons, Ecosystèmes et Aquaculture Durable en Nouvelle-Calédonie, BP 2059, 98846 Nouméa cedex, New Caledonia C2 UNIV MED S CAROLINA, USA UNIV MED S CAROLINA, USA MARINE RESOURCES INST, USA IFREMER, FRANCE SI SAINT VINCENT SE PDG-DOP-DCOP-LEADNC-LAC IN WOS Ifremer jusqu'en 2018 copubli-int-hors-europe IF 3.293 TC 66 UR https://archimer.ifremer.fr/doc/00015/12652/9587.pdf LA English DT Article DE ;Shrimp;Litopenaeus vannamei;RNAi;dsRNA;Argonaute;Sid-1;Antiviral immunity AB Many questions remain unanswered regarding RNAi-based mechanisms and dsRNA-induced antiviral immune responses in penaeid shrimp. In this study, we report the characterization in the white leg shrimp Litopenaeus vannamei of RNAi pathway associated proteins Lv-Ago 1 and Lv-Ago 2, two members of the Argonaute family of proteins, as well as Lv-sid 1, the first shrimp homologue of Sid-1, a membrane channel-forming protein implicated in the cellular import of dsRNA. To decipher their functional implication in RNAi-related phenomena, we monitored their relative expression following stimulation by specific and non-specific RNA duplexes of diverse length. The findings show that the length of small RNA duplexes plays a critical role in the activation of both RNAi-related and innate antiviral responses. They also suggest that these two mechanisms of antiviral response may activate the same pathway, requiring Lv-Sid 1 and Lv-Ago 2 induction. (C) 2010 Elsevier Ltd. All rights reserved. PY 2010 PD NOV SO Developmental And Comparative Immunology SN 0145-305X PU Elsevier Sci Ltd VL 34 IS 11 UT 000282558800009 BP 1209 EP 1218 DI 10.1016/j.dci.2010.06.017 ID 12652 ER EF