FN Archimer Export Format
PT J
TI Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France
BT 
AF AZANDEGBE, Afi
   GARNIER, Matthieu
   ANDRIEUX, Francoise
   KEROUEL, Roger
   PHILIPPON, Xavier
   NICOLAS, Jean-Louis
AS 1:1;2:1;3:2;4:2;5:2;6:1;
FF 1:PDG-DOP-DCB-PFOM-PI;2:;3:PDG-DOP-DCB-DYNECO-PELAGOS;4:PDG-DOP-DCB-DYNECO-PELAGOS;5:PDG-DOP-DCB-DYNECO-PELAGOS;6:PDG-DOP-DCB-PFOM-PI;
C1 PFOM, Lab Physiol Invertebres, F-29280 Plouzane, France.
   IFREMER, Ctr Brest, Lab Dyneco, F-29280 Plouzane, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-DOP-DCB-PFOM-PI
   PDG-DOP-DCB-DYNECO-PELAGOS
IN WOS Ifremer jusqu'en 2018
IF 1.572
TC 25
UR https://archimer.ifremer.fr/doc/00017/12813/9756.pdf
LA English
DT Article
DE ;Vibrio aestuarianus;Crassostrea gigas Sediment;Seasonal abundance;Colony-blot
AB Vibrio aestuarianus is frequently found in coastal areas and can infect and induce mortalities in the pacific oyster Crassostrea gigas. However, nothing is known about its distribution and seasonality in the estuarine environment, especially where oyster farming is practiced. Its occurrence was investigated in sediment and oyster haemolymph at 2 oyster farms in Brittany (France) over 2 yr during 2 periods, from June to September 2007 and from February to June 2008. Total heterotrophic bacteria (HB) were cultured on marine agar while total Vibrio spp. and V. aestuarianus were selectively numerated using thiosulfate citrate bile salts sucrose agar (TCBS agar) and the species-specific hybridisation method, respectively. PCR was performed to detect V. aestuarianus in sediment when it became unculturable. Both total Vibrio spp. and V. aestuarianus had a seasonal trend. The highest concentrations were recovered in the warmest months. Its abundance ranged from 10(2) to 4 x 10(5) CFU ml(-1) in haemolymph and from 10(3) to 1 x 10(4) CFU g(-1) in the sediment. Temperature was the main factor influencing the concentration of Vibrio spp. and V. aestuarianus in the sediment. Thus V aestuarianus might subsist during the cold seasons in the sediment, from which it can emerge when environmental conditions became favourable.
PY 2010
PD SEP
SO Diseases Of Aquatic Organisms
SN 0177-5103
PU Inter-research
VL 91
IS 3
UT 000283571500004
BP 213
EP 221
DI 10.3354/dao02253
ID 12813
ER

EF