FN Archimer Export Format
PT J
TI Molecular responses of Ostrea edulis haemocytes to an in vitro infection with Bonamia ostreae
BT 
AF MORGA, Benjamin
   ARZUL, Isabelle
   FAURY, Nicole
   SEGARRA, Amelie
   CHOLLET, Bruno
   RENAULT, Tristan
AS 1:1;2:1;3:1;4:1;5:1;6:1;
FF 1:PDG-DOP-DCN-AGSAE-LGP;2:PDG-RBE-AGSAE-LGP;3:PDG-RBE-AGSAE-LGP;4:PDG-RBE-AGSAE-LGP;5:PDG-RBE-AGSAE-LGP;6:PDG-RBE-AGSAE-LGP;
C1 Inst Francais Rech Exploitat Mer IFREMER, LGP, F-17390 La Tremblade, France.
C2 IFREMER, FRANCE
SI LA TREMBLADE
SE PDG-DOP-DCN-AGSAE-LGP
   PDG-RBE-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
IF 3.268
TC 44
UR https://archimer.ifremer.fr/doc/00018/12879/9828.pdf
LA English
DT Article
DE ;Bonamia ostreae;Protozoan;Ostrea edulis;Flat oyster;Haemocytes;Gene expression;Suppression subtractive hybridisation
AB Bonamiosis due to the parasite Bonamia ostreae is a disease affecting the flat oyster Ostrea edulis. B. ostreae is a protozoan, affiliated to the order of haplosporidia and to the cercozoan phylum. This parasite is mainly intracellular, infecting haemocytes, cells notably involved in oyster defence mechanisms.  Suppression subtractive hybridisation (SSH) was carried out in order to identify oyster genes differentially expressed during an infection of haemocytes with B. ostreae. Forward and reverse banks allowed obtaining 1104 and 1344 clones respectively, among which 391 and 480 clones showed a differential expression between both tested conditions (haemocytes alone versus haemocytes in contact with parasites). ESTs of interest including genes involved in cytoskeleton, respiratory chain, detoxification membrane receptors, and immune system were identified. The open reading frames of two selected genes (galectin and IRF-like) were completely sequenced and characterized. Real time PCR assays were developed to study the relative expression of candidate ESTs during an in vitro infection of haemocytes by live and dead parasites.  Haemocyte infection with B. ostreae induced an increased expression of omega glutathione S-transferase (OGST), superoxide dismutase (SOD), tissue inhibitor of metalloproteinase (TIMP), galectin, interferon regulatory factor (IRF-like) and filamin genes.
PY 2011
PD MAR
SO Developmental And Comparative Immunology
SN 0145-305X
PU Elsevier Sci Ltd
VL 35
IS 3
UT 000289871800012
BP 323
EP 333
DI 10.1016/j.dci.2010.10.005
ID 12879
ER

EF