The toxicity of benzo[a]pyrene on sole (Solea solea) hepatocytes: assessment of genotoxic and enzymatic effects
|Author(s)||Wessel Nathalie1, Menard Dominique1, Pichavant-Rafini K.2, Ollivier H.2, Le Goff Jean-Michel3, Burgeot Thierry1, Akcha Farida1|
|Affiliation(s)||1 : IFREMER, Dept Biogeochem & Ecotoxicol, F-443110 Nantes 03, France.
2 : Univ Brest, Univ Europeenne Bretagne, Lab Optimisat Regulat Physiol, F-29238 Brest, France.
3 : Univ Caen Basse Normandie, ADntox & Grp Reg Etudes CANc, Ctr Francois Baclesse, F-14076 Caen 05, France.
|Source||Polycyclic Aromatic Compounds (1040-6638) (Taylor & Francis Ltd), 2010 , Vol. 30 , N. 5 , P. 346-354|
|WOS© Times Cited||7|
|Keyword(s)||Genotoxicity, Sole, BaP, DNA strand breaks, EROD, DNA adducts|
|Abstract||The benzo[a]pyrene is a polycyclic aromatic hydrocarbon known to be genotoxic, mutagenic and carcinogenic in higher vertebrates. The aim of this study was to evaluate in vitro the enzymatic and genotoxic effects of BaP in a benthic fish species, Solea solea. Sole hepatocytes were exposed to BaP in order to measure the modulation of ethoxyresorufin-o-deethylase (EROD) activity and the DNA strand breaks induced by BaP metabolism. Exposures were performed in both culture flasks and microplate wells in order to check for the possible miniaturization of the exposure system. Moreover, sole liver microsomes were exposed to BaP in the presence of standard DNA in order to assess the potential formation of DNA adducts in sole. The results demonstrated the ability of sole hepatic enzymes to metabolize BaP into reactive species responsible for bulky DNA adducts and DNA strand breakage, whatever the tested exposure concentration and the mode of exposure.|