FN Archimer Export Format
PT J
TI The toxicity of benzo[a]pyrene on sole (Solea solea) hepatocytes: assessment of genotoxic and enzymatic effects
BT 
AF WESSEL, Nathalie
   MENARD, Dominique
   PICHAVANT-RAFINI, K.
   OLLIVIER, H.
   LE GOFF, Jean-Michel
   BURGEOT, Thierry
   AKCHA, Farida
AS 1:1;2:1;3:2;4:2;5:3;6:1;7:1;
FF 1:PDG-DOP-DCN-BE-LBEX;2:PDG-DOP-DCN-BE-LBEX;3:;4:;5:;6:PDG-DOP-DCN-BE-LBEX;7:PDG-DOP-DCN-BE-LBEX;
C1 IFREMER, Dept Biogeochem & Ecotoxicol, F-443110 Nantes 03, France.
   Univ Brest, Univ Europeenne Bretagne, Lab Optimisat Regulat Physiol, F-29238 Brest, France.
   Univ Caen Basse Normandie, ADntox & Grp Reg Etudes CANc, Ctr Francois Baclesse, F-14076 Caen 05, France.
C2 IFREMER, FRANCE
   UBO, FRANCE
   UNIV CAEN, FRANCE
SI NANTES
SE PDG-DOP-DCN-BE-LBEX
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 0.982
TC 7
UR https://archimer.ifremer.fr/doc/00018/12968/9944.pdf
LA English
DT Article
DE ;Genotoxicity;Sole;BaP;DNA strand breaks;EROD;DNA adducts
AB The benzo[a]pyrene is a polycyclic aromatic hydrocarbon known to be genotoxic, mutagenic and carcinogenic in higher vertebrates. The aim of this study was to evaluate in vitro the enzymatic and genotoxic effects of BaP in a benthic fish species, Solea solea. Sole hepatocytes were exposed to BaP in order to measure the modulation of ethoxyresorufin-o-deethylase (EROD) activity and the DNA strand breaks induced by BaP metabolism. Exposures were performed in both culture flasks and microplate wells in order to check for the possible miniaturization of the exposure system. Moreover, sole liver microsomes were exposed to BaP in the presence of standard DNA in order to assess the potential formation of DNA adducts in sole. The results demonstrated the ability of sole hepatic enzymes to metabolize BaP into reactive species responsible for bulky DNA adducts and DNA strand breakage, whatever the tested exposure concentration and the mode of exposure.
PY 2010
SO Polycyclic Aromatic Compounds
SN 1040-6638
PU Taylor & Francis Ltd
VL 30
IS 5
UT 000284418800009
BP 346
EP 354
ID 12968
ER

EF