Relationship between PAH biotransformation as measured by biliary metabolites and EROD activity, and genotoxicity in juveniles of sole (Solea solea)
|Author(s)||Wessel Nathalie1, Santos Raphael1, Menard Dominique1, Le Menach K.3, Buchet Vincent2, Le Bayon Nicolas2, Loizeau Veronique1, Burgeot Thierry1, Budzinski H.3, Akcha Farida1|
|Affiliation(s)||1 : IFREMER, Dept Biogeochem & Ecotoxicol, F-44311 Nantes 03, France.
2 : IFREMER, Lab Adaptat Reprod & Nutr Marine Fish, F-29280 Plouzane, France.
3 : Univ Bordeaux 1, ISM, LPTC, F-33405 Talence, France.
|Meeting||15th International Symposium on Pollutant Responses in Marine Organisms (PRIMO15), Bordeaux, FRANCE, MAY 17-20, 2009|
|Source||Marine Environmental Research (0141-1136) (Elsevier Sci Ltd), 2010 , Vol. 69 , P. S71-S73|
|WOS© Times Cited||49|
|Keyword(s)||Ecotoxicology, Sole, PAH, Metabolites, EROD, Comet assay|
|Abstract||Polycylic aromatic hydrocarbons (PAHs) are ubiquitous contaminants in the marine environment Their toxicity is mainly linked to the ability of marine species to biotransform them into reactive metabolites PAHs are thus often detected at trace levels in animal tissues For biomonitoring purposes this findings have two main consequences (i) the determination of the PAH tissue concentration is not suitable for the evaluation of individual exposure to PAHs (ii) It can explain sometimes the lack of correlations obtained with relevant markers of toxicity such as genotoxicity biomarkers The aim of the present study was to better investigate the link between PAH exposure and genotoxicity in marine flatfish During a laboratory experiment juvenile soles were exposed for four weeks to a mixture of three PAHs namely benzo[a]pyrene fluoranthene and pyrene followed by one week of depuration Fish were exposed via the trophic route to a daily PAH concentration of 120 mu g/g food Fish were sampled at different time points The bioavailability and the biotransformation of PAHs were assessed by the measurement of biliary metabolites using a sensitive UPLC MS/MS method The 7-ethoxyresorufine-O-deethylase was also measured in liver subcellular fractions as a biomarker of phase I biotransformation activities Genotoxicity was assessed in parallel by the measurement of DNA strand breaks in fish erythrocytes by the alkaline comet assay During this study the high amount of PAH metabolites produced in sole demonstrated the bioavailability of PAHs and their biotransformation by fish enzymes A positive correlation was observed between the level of hydroxylated PAH metabolites and genotoxicity as measured by the alkaline comet assay (C) 2010 Elsevier Ltd All rights reserved|