Adaptation to Cold and Proteomic Responses of the Psychrotrophic Biopreservative Lactococcus piscium Strain CNCM I-4031

Type Article
Date 2010-12
Language English
Author(s) Garnier MatthieuORCID1, 5, Matamoros Sebastien1, 3, 4, Chevret Didier2, Pilet Marie-France1, 3, 4, Leroi Francoise1, Tresse Odile3, 4
Affiliation(s) 1 : Lab BRM STBM Ifremer, Nantes, France.
2 : PAPPSO, Inst MICALIS, UMR 1319, Jouy En Josas, France.
3 : INRA, SECALIM, UMR 1014, F-44026 Nantes, France.
4 : ONIRIS, Nantes, France.
5 : Lab BRM PBA Ifremer, Nantes, France.
Source Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 2010-12 , Vol. 76 , N. 24 , P. 8011-8018
DOI 10.1128/AEM.01331-10
WOS© Times Cited 26
Abstract There is considerable interest in the use of psychrotrophic bacteria for food biopreservation and in the understanding of cold adaptation mechanisms. The psychrotrophic biopreservative Lactococcus piscium strain CNCM I-4031 was studied for its growth behavior and proteomic responses after cold shock and during cold acclimation. Growth kinetics highlighted the absence of growth latency after cold shock, suggesting a very high promptness in cold adaptation, a behavior that has never been described before for lactic acid bacteria (LAB). A comparative proteomic analysis was applied with two-dimensional gel electrophoresis (2-DE), and upregulated proteins were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Both cold shock and cold acclimation triggered the upregulation of proteins involved in general and oxidative stress responses and fatty acid and energetic metabolism. However, 2-DE profiles and upregulated proteins were different under both conditions, suggesting a sequence of steps in cold adaptation. In addition, the major 7-kDa Csp protein was identified in the L. piscium CNCM I-4031 genome but was not cold regulated. The implication of the identified cold shock proteins and cold acclimation proteins in efficient cold adaptation, the possible regulation of a histidyl phosphocarrier protein, and the roles of a constitutive major 7-kDa Csp are discussed.
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Garnier Matthieu, Matamoros Sebastien, Chevret Didier, Pilet Marie-France, Leroi Francoise, Tresse Odile (2010). Adaptation to Cold and Proteomic Responses of the Psychrotrophic Biopreservative Lactococcus piscium Strain CNCM I-4031. Applied And Environmental Microbiology, 76(24), 8011-8018. Publisher's official version : https://doi.org/10.1128/AEM.01331-10 , Open Access version : https://archimer.ifremer.fr/doc/00019/13047/