Adaptation to Cold and Proteomic Responses of the Psychrotrophic Biopreservative Lactococcus piscium Strain CNCM I-4031
|Author(s)||Garnier Matthieu1, 5, Matamoros Sebastien1, 3, 4, Chevret Didier2, Pilet Marie-France1, 3, 4, Leroi Francoise1, Tresse Odile3, 4|
|Affiliation(s)||1 : Lab BRM STBM Ifremer, Nantes, France.
2 : PAPPSO, Inst MICALIS, UMR 1319, Jouy En Josas, France.
3 : INRA, SECALIM, UMR 1014, F-44026 Nantes, France.
4 : ONIRIS, Nantes, France.
5 : Lab BRM PBA Ifremer, Nantes, France.
|Source||Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 2010-12 , Vol. 76 , N. 24 , P. 8011-8018|
|WOS© Times Cited||27|
|Abstract||There is considerable interest in the use of psychrotrophic bacteria for food biopreservation and in the understanding of cold adaptation mechanisms. The psychrotrophic biopreservative Lactococcus piscium strain CNCM I-4031 was studied for its growth behavior and proteomic responses after cold shock and during cold acclimation. Growth kinetics highlighted the absence of growth latency after cold shock, suggesting a very high promptness in cold adaptation, a behavior that has never been described before for lactic acid bacteria (LAB). A comparative proteomic analysis was applied with two-dimensional gel electrophoresis (2-DE), and upregulated proteins were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Both cold shock and cold acclimation triggered the upregulation of proteins involved in general and oxidative stress responses and fatty acid and energetic metabolism. However, 2-DE profiles and upregulated proteins were different under both conditions, suggesting a sequence of steps in cold adaptation. In addition, the major 7-kDa Csp protein was identified in the L. piscium CNCM I-4031 genome but was not cold regulated. The implication of the identified cold shock proteins and cold acclimation proteins in efficient cold adaptation, the possible regulation of a histidyl phosphocarrier protein, and the roles of a constitutive major 7-kDa Csp are discussed.|