Adaptation to Cold and Proteomic Responses of the Psychrotrophic Biopreservative Lactococcus piscium Strain CNCM I-4031
| Type | Article | ||||||||||||
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| Date | 2010-12 | ||||||||||||
| Language | English | ||||||||||||
| Author(s) | Garnier Matthieu 1, 5, Matamoros Sebastien1, 3, 4, Chevret Didier2, Pilet Marie-France1, 3, 4, Leroi Francoise1, Tresse Odile3, 4 |
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| Affiliation(s) | 1 : Lab BRM STBM Ifremer, Nantes, France. 2 : PAPPSO, Inst MICALIS, UMR 1319, Jouy En Josas, France. 3 : INRA, SECALIM, UMR 1014, F-44026 Nantes, France. 4 : ONIRIS, Nantes, France. 5 : Lab BRM PBA Ifremer, Nantes, France. |
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| Source | Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 2010-12 , Vol. 76 , N. 24 , P. 8011-8018 | ||||||||||||
| DOI | 10.1128/AEM.01331-10 | ||||||||||||
| WOS© Times Cited | 27 | ||||||||||||
| Abstract | There is considerable interest in the use of psychrotrophic bacteria for food biopreservation and in the understanding of cold adaptation mechanisms. The psychrotrophic biopreservative Lactococcus piscium strain CNCM I-4031 was studied for its growth behavior and proteomic responses after cold shock and during cold acclimation. Growth kinetics highlighted the absence of growth latency after cold shock, suggesting a very high promptness in cold adaptation, a behavior that has never been described before for lactic acid bacteria (LAB). A comparative proteomic analysis was applied with two-dimensional gel electrophoresis (2-DE), and upregulated proteins were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Both cold shock and cold acclimation triggered the upregulation of proteins involved in general and oxidative stress responses and fatty acid and energetic metabolism. However, 2-DE profiles and upregulated proteins were different under both conditions, suggesting a sequence of steps in cold adaptation. In addition, the major 7-kDa Csp protein was identified in the L. piscium CNCM I-4031 genome but was not cold regulated. The implication of the identified cold shock proteins and cold acclimation proteins in efficient cold adaptation, the possible regulation of a histidyl phosphocarrier protein, and the roles of a constitutive major 7-kDa Csp are discussed. | ||||||||||||
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