FN Archimer Export Format
PT J
TI Adaptation to Cold and Proteomic Responses of the Psychrotrophic Biopreservative Lactococcus piscium Strain CNCM I-4031
BT 
AF GARNIER, Matthieu
   MATAMOROS, Sebastien
   CHEVRET, Didier
   PILET, Marie-France
   LEROI, Francoise
   TRESSE, Odile
AS 1:1,5;2:1,3,4;3:2;4:1,3,4;5:1;6:3,4;
FF 1:PDG-DOP-DCN-BRM-PBA;2:PDG-DOP-DCN-STAM;3:;4:;5:PDG-DOP-DCN-BRM-STBM;6:;
C1 Lab BRM STBM Ifremer, Nantes, France.
   PAPPSO, Inst MICALIS, UMR 1319, Jouy En Josas, France.
   INRA, SECALIM, UMR 1014, F-44026 Nantes, France.
   ONIRIS, Nantes, France.
   Lab BRM PBA Ifremer, Nantes, France.
C2 IFREMER, FRANCE
   INRA, FRANCE
   INRA, FRANCE
   ONIRIS, FRANCE
   IFREMER, FRANCE
SI NANTES
SE PDG-DOP-DCN-BRM-PBA
   PDG-DOP-DCN-STAM
   PDG-DOP-DCN-BRM-STBM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
IF 3.778
TC 27
UR https://archimer.ifremer.fr/doc/00019/13047/10150.pdf
LA English
DT Article
AB There is considerable interest in the use of psychrotrophic bacteria for food biopreservation and in the understanding of cold adaptation mechanisms. The psychrotrophic biopreservative Lactococcus piscium strain CNCM I-4031 was studied for its growth behavior and proteomic responses after cold shock and during cold acclimation. Growth kinetics highlighted the absence of growth latency after cold shock, suggesting a very high promptness in cold adaptation, a behavior that has never been described before for lactic acid bacteria (LAB). A comparative proteomic analysis was applied with two-dimensional gel electrophoresis (2-DE), and upregulated proteins were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Both cold shock and cold acclimation triggered the upregulation of proteins involved in general and oxidative stress responses and fatty acid and energetic metabolism. However, 2-DE profiles and upregulated proteins were different under both conditions, suggesting a sequence of steps in cold adaptation. In addition, the major 7-kDa Csp protein was identified in the L. piscium CNCM I-4031 genome but was not cold regulated. The implication of the identified cold shock proteins and cold acclimation proteins in efficient cold adaptation, the possible regulation of a histidyl phosphocarrier protein, and the roles of a constitutive major 7-kDa Csp are discussed.
PY 2010
PD DEC
SO Applied And Environmental Microbiology
SN 0099-2240
PU Amer Soc Microbiology
VL 76
IS 24
UT 000284882800015
BP 8011
EP 8018
DI 10.1128/AEM.01331-10
ID 13047
ER

EF