FN Archimer Export Format
PT C
TI Discrimination between human, pig and ruminant fecal contaminations in a river catchment by real-time PCR using host-specific markers
BT 
AF MIESZKIN, Sophie
   FURET, Jean-Pierre
   CORTHIER, Gerard
   POMMEPUY, Monique
   LE SAUX, Jean-Claude
   BOUGEARD, Morgane
   HERVIO HEATH, Dominique
   GOURMELON, Michele
AS 1:1;2:2;3:2;4:1;5:1;6:1;7:1;8:1;
FF 1:PDG-DOP-DCN-EMP-MIC;2:;3:;4:PDG-DOP-DCN-EMP-MIC;5:PDG-DOP-DCN-EMP-MIC;6:PDG-DOP-LER-LERN;7:PDG-DOP-DCN-EMP-MIC;8:PDG-DOP-DCN-EMP-MIC;
C1 Ifremer EMP, Laboratoire de Microbiologie, 29280 Plouzané, France
   Unité d’écologie et de physiologie du système digestif (UEPSD), INRA, 78352 Jouy-en-Josas cedex, France
C2 IFREMER, FRANCE
   INRA, FRANCE
SI BREST
   PORT-EN-BESSIN
SE PDG-DOP-DCN-EMP-MIC
   PDG-DOP-LER-LERN
UR https://archimer.ifremer.fr/doc/00021/13259/10306.pdf
LA English
DT Proceedings paper
DE ;Microbial Source Tracking;Host-Specific Bacteroidales Marker;16S rRNA Gene;Real-Time PCR;Faecal Contamination
AB The microbiological quality of coastal waters and shellfish harvesting areas in Brittany (France) can be affected by faecal pollutions from human activities and animal breeding (especially pigs and cattle). To discriminate among faecal pollution of human and animal origin, a library-independent microbial source tracking method was selected: Bacteroidales host-specific 16S rRNA gene markers by real-time Polymerase Chain Reaction (PCR). A human-specific Bacteroidales marker (Hum-1-Bac) was designed. Tested on faecal samples, the Hum-1-Bac marker showed 95 % sensitivity and 95 % specificity (n= 80). Average values (± STD) of the Hum-1-Bac marker were found to be 7.3 ± 1.4 16S rRNA gene copies per g wet faeces in human faeces samples (n=10) and 5.7 ± 1.3 log10 copies per 100 ml water in Sewage Treatment Plant (STP) water samples (n=10). These results highlight that the human-specific marker present in individual faeces was still present in the effluents. The human-specific marker developed in this study (Hum-1-Bac) and the human- (HF183), pig- (Pig-2-Bac) and ruminant- (Rum-2-Bac) specific Bacteroidales markers previously described by Seurinck et al. (2005) and Mieszkin et al. (2009, 2010) were then applied to river water samples (n=33) collected in 2008-2009 from the catchment of the Daoulas river estuary (Brittany, France), upstream of shellfish harvesting areas. For all sites, at least two host-specific markers were detected. The ruminant-specific Bacteroidales marker was more often quantified (60.6 %) than the human- or pig-specific Bacteroidales markers (Hum-1-Bac, 45.5 %; HF183, 48.5 % and Pig-2-Bac, 30.3 %) in river water samples. These results show that faecal pollution came generally from multiple origins and that host-specific Bacteroidales markers are promising as a quantitative microbial source tracking method to determine sources of faecal pollution in environmental water.
PY 2009
PD JUL
CT ICMSS09 – Nantes, France – June 2009
ID 13259
ER

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