Experimental ostreid herpesvirus 1 infection of the Pacific oyster Crassostrea gigas: Kinetics of virus DNA detection by q-PCR in seawater and in oyster samples

Type Article
Date 2011-01
Language English
Author(s) Schikorski David1, Faury Nicole1, Pepin Jean-Francois1, Saulnier Denis1, Tourbiez Delphine1, Renault Tristan1
Affiliation(s) 1 : IFREMER, LGP, F-17390 La Tremblade, France.
Source Virus Research (0168-1702) (Elsevier Science Bv), 2011-01 , Vol. 155 , N. 1 , P. 28-34
DOI 10.1016/j.virusres.2010.07.031
WOS© Times Cited 108
Keyword(s) Ostreid herpesvirus 1, Cupped Pacific oyster, Crassostrea gigas, Experimental infection, Kinetic viral DNA quantification
Abstract Herpes- and herpes-like viruses are known to infect a wide range of bivalve mollusc species throughout the world. Abnormal summer mortalities associated to the detection of ostreid herpesvirus 1 (OsHV-1) have been currently reported in France among larvae and spat of the Pacific cupped oyster Crassostrea gigas.

In the present work, we have developed an experimental protocol of horizontal transmission based on the cohabitation between healthy and experimentally infected oysters. Through a cohabitation trial, the kinetics of OsHV-1 detection in different oyster organs and seawater samples were investigated and characterized for the first time using real time quantitative PCR.
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Schikorski David, Faury Nicole, Pepin Jean-Francois, Saulnier Denis, Tourbiez Delphine, Renault Tristan (2011). Experimental ostreid herpesvirus 1 infection of the Pacific oyster Crassostrea gigas: Kinetics of virus DNA detection by q-PCR in seawater and in oyster samples. Virus Research, 155(1), 28-34. Publisher's official version : https://doi.org/10.1016/j.virusres.2010.07.031 , Open Access version : https://archimer.ifremer.fr/doc/00025/13607/