TY - JOUR T1 - Experimental ostreid herpesvirus 1 infection of the Pacific oyster Crassostrea gigas: Kinetics of virus DNA detection by q-PCR in seawater and in oyster samples A1 - Schikorski,David A1 - Faury,Nicole A1 - Pepin,Jean-Francois A1 - Saulnier,Denis A1 - Tourbiez,Delphine A1 - Renault,Tristan AD - IFREMER, LGP, F-17390 La Tremblade, France. UR - https://doi.org/10.1016/j.virusres.2010.07.031 DO - 10.1016/j.virusres.2010.07.031 KW - Ostreid herpesvirus 1 KW - Cupped Pacific oyster KW - Crassostrea gigas KW - Experimental infection KW - Kinetic viral DNA quantification N2 - Herpes- and herpes-like viruses are known to infect a wide range of bivalve mollusc species throughout the world. Abnormal summer mortalities associated to the detection of ostreid herpesvirus 1 (OsHV-1) have been currently reported in France among larvae and spat of the Pacific cupped oyster Crassostrea gigas. In the present work, we have developed an experimental protocol of horizontal transmission based on the cohabitation between healthy and experimentally infected oysters. Through a cohabitation trial, the kinetics of OsHV-1 detection in different oyster organs and seawater samples were investigated and characterized for the first time using real time quantitative PCR. Y1 - 2011/01 PB - Elsevier Science Bv JF - Virus Research SN - 0168-1702 VL - 155 IS - 1 SP - 28 EP - 34 ID - 13607 ER -