FN Archimer Export Format PT J TI Experimental ostreid herpesvirus 1 infection of the Pacific oyster Crassostrea gigas: Kinetics of virus DNA detection by q-PCR in seawater and in oyster samples BT AF SCHIKORSKI, David FAURY, Nicole PEPIN, Jean-Francois SAULNIER, Denis TOURBIEZ, Delphine RENAULT, Tristan AS 1:1;2:1;3:1;4:1;5:1;6:1; FF 1:PDG-RBE-AGSAE-LGP;2:PDG-RBE-AGSAE-LGP;3:PDG-RBE-AGSAE-LGP;4:PDG-RBE-AQUAPOL-LBQP;5:PDG-RBE-AGSAE-LGP;6:PDG-RBE-AGSAE-LGP; C1 IFREMER, LGP, F-17390 La Tremblade, France. C2 IFREMER, FRANCE SI BREST LA TREMBLADE TAHITI SE PDG-RBE-AGSAE-LGP PDG-RBE-AQUAPOL-LBQP IN WOS Ifremer jusqu'en 2018 IF 2.941 TC 122 UR https://archimer.ifremer.fr/doc/00025/13607/10688.pdf LA English DT Article DE ;Ostreid herpesvirus 1;Cupped Pacific oyster;Crassostrea gigas;Experimental infection;Kinetic viral DNA quantification AB Herpes- and herpes-like viruses are known to infect a wide range of bivalve mollusc species throughout the world. Abnormal summer mortalities associated to the detection of ostreid herpesvirus 1 (OsHV-1) have been currently reported in France among larvae and spat of the Pacific cupped oyster Crassostrea gigas. In the present work, we have developed an experimental protocol of horizontal transmission based on the cohabitation between healthy and experimentally infected oysters. Through a cohabitation trial, the kinetics of OsHV-1 detection in different oyster organs and seawater samples were investigated and characterized for the first time using real time quantitative PCR. PY 2011 PD JAN SO Virus Research SN 0168-1702 PU Elsevier Science Bv VL 155 IS 1 UT 000287010300004 BP 28 EP 34 DI 10.1016/j.virusres.2010.07.031 ID 13607 ER EF