FN Archimer Export Format PT J TI Effect of food supply on the detoxification in the blue mussel, Mytilus edulis, contaminated by diarrhetic shellfish toxins BT AF MARCAILLOU-LE BAUT, Claire HAURE, Joel MONDEGUER, Florence COURCOUX, Anne DUPUY, Beatrice PENISSON, Christian AS 1:1;2:2;3:1;4:1;5:2;6:2; FF 1:PDG-DOP-DCN-EMP-PHYC;2:PDG-DOP-DCN-AGSAE-LGP;3:PDG-DOP-DCN-EMP-PHYC;4:PDG-DOP-DCN-EMP-PHYC;5:PDG-DOP-DCN-AGSAE-LGP;6:PDG-DOP-DCN-AGSAE-LGP; C1 IFREMER, Dep Environm Microbiol & Phycotoxines, F-44311 Nantes, France. IFREMER, Stn Conchylicole Pays Loire, F-85230 Bouin, France. C2 IFREMER, FRANCE IFREMER, FRANCE SI NANTES BOUIN SE PDG-DOP-DCN-EMP-PHYC PDG-DOP-DCN-AGSAE-LGP IN WOS Ifremer jusqu'en 2018 IF 1.062 TC 17 UR https://archimer.ifremer.fr/doc/00025/13650/10883.pdf LA English DT Article DE ;Mussel;Feeding;Digestive gland;Depuration;Okadaic acid;Acyl esters;Marine lipophilic biotoxins;Mass spectrometry AB The objective of this study was to identify a possible effect of food on the acceleration of decontamination in blue mussels, Mytilus edulis, containing diarrhetic toxins belonging to the okadaic acid (OA) structural group. An experimental protocol was designed to describe and compare the decontamination kinetics and detoxification rates of naturally OA-contaminated mussels that had either received or not received food for three weeks. The protocol was applied in two trials (in June 2006 and June 2007, called Ker06 and Ker07), conducted one year apart on samples of mussels collected in the same area, at the same season. Okadaic acid (OA), the main lipophilic toxin produced by the toxic alga Dinophysis acuminata, was analysed over the course of the decontamination, in hydrolysed (total OA) and non hydrolysed (free OA) digestive gland extracts, in order to estimate acyl-esters (7-O-acyl-ester derivatives of OA) concentrations. OA analyses were also made for toxin presence in biodeposits and in the aqueous phase. Bivalve physiological status was evaluated by biomass measurements (dry weight of flesh) and two biochemical compounds (total lipid and glycogen concentrations). Measured physiological parameters showed that mussels did not suffer under experimental conditions. Both trials showed that the food accelerated the elimination of free and total OA. This effect cannot be attributed to a dilution of the toxin in the tissue, as results expressed in toxin burden led to the same conclusion. The kinetic models of decontamination differed between the two experiments: the model was linear for Ker06, provided that the values corresponding to the first two days were discarded, whereas data fitted a decreasing exponential curve better in Ker07. Detoxification rates increased as the food supply increased. After three weeks of experimentation, the detoxification rates for total OA in Ker06 and Ker07 were 52 and 61%, respectively, in unfed mussels and 90 and 89% in fed mussels (with the highest level in Ker07). Comparisons between the free OA and esters showed that detoxification rate was higher for free OA than for esters, whatever the level of food supply. The results of this study suggest that food increase probably accelerates elimination of OA and OA-esters but the latter are eliminated at a lower speed. PY 2010 PD JUN SO Aquatic Living Resources SN 0990-7440 PU Edp Sciences S A VL 23 IS 3 UT 000285629500003 BP 255 EP 266 DI 10.1051/alr/2010026 ID 13650 ER EF