FN Archimer Export Format
PT J
TI Sterilization of Exopolysaccharides Produced by Deep-Sea Bacteria: Impact on Their Stability and Degradation
BT 
AF REDERSTORFF, Emilie
   FATIMI, Ahmed
   SINQUIN, Corinne
   RATISKOL, Jacqueline
   MERCERON, Christophe
   VINATIER, Claire
   WEISS, Pierre
   COLLIEC-JOUAULT, Sylvia
AS 1:1,2;2:2;3:1;4:1;5:2;6:2;7:2;8:1;
FF 1:;2:;3:PDG-RBE-BRM-BMM;4:PDG-RBE-BRM-BMM;5:PDG-DOP-DCN-STAM;6:;7:;8:PDG-RBE-BRM-BMM;
C1 French Res Inst Exploitat Sea IFREMER, Lab Biotechnol & Marine Mol, F-44311 Nantes 03, France.
   Univ Nantes, INSERM UMRS 791, Lab Osteoarticular & Dent Tissue Engn, Sch Dent Surg, F-44042 Nantes 1, France.
C2 IFREMER, FRANCE
   UNIV NANTES, FRANCE
SI NANTES
SE PDG-RBE-BRM-BMM
   PDG-DOP-DCN-STAM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.854
TC 9
UR https://archimer.ifremer.fr/doc/00027/13776/10913.pdf
LA English
DT Article
DE ;marine biotechnology;polysaccharides;sterilization procedures;characterization;molecular weight distribution;rheology
AB Polysaccharides are highly heat-sensitive macromolecules, so high temperature treatments are greatly destructive and cause considerable damage, such as a great decrease in both viscosity and molecular weight of the polymer. The technical feasibility of the production of exopolysaccharides by deep-sea bacteria Vibrio diabolicus and Alteromonas infernus was previously demonstrated using a bioproduct manufacturing process. The objective of this study was to determine which sterilization method, other than heat sterilization, was the most appropriate for these marine exopolysaccharides and was in accordance with bioprocess engineering requirements. Chemical sterilization using low-temperature ethylene oxide and a mixture of ionized gases (plasmas) was compared to the sterilization methods using gamma and beta radiations. The changes to both the physical and chemical properties of the sterilized exopolysaccharides were analyzed. The use of ethylene oxide can be recommended for the sterilization of polysaccharides as a weak effect on both rheological and structural properties was observed. This low-temperature gas sterilizing process is very efficient, giving a good Sterility Assurance Level (SAL), and is also well suited to large-scale compound manufacturing in the pharmaceutical industry.
PY 2011
PD FEB
SO Marine Drugs
SN 1660-3397
PU Mdpi Ag
VL 9
IS 2
UT 000288892400005
BP 224
EP 241
DI 10.3390/md9020224
ID 13776
ER

EF