FN Archimer Export Format PT J TI Calcein staining of calcified structures in pearl oyster Pinctada margaritifera and the effect of food resource level on shell growth BT AF LINARD, Clementine GUEGUEN, Yannick MORICEAU, Jacques SOYEZ, Claude HUI, Belinda RAOUX, Aurore CUIF, Jean Pierre COCHARD, Jean-Claude LE PENNEC, Marcel LE MOULLAC, Gilles AS 1:1,3;2:2;3:1;4:1;5:1;6:1;7:4;8:1,5;9:1,3;10:1; FF 1:;2:PDG-RBE-AQUAPOL-LBQP;3:PDG-RBE-PFOM-ARN;4:PDG-RBE-AQUAPOL-LDHP;5:PDG-DOP-DCOP-AQUAPOL-LDHP;6:PDG-DOP-DCOP-AQUAPOL-LDHP;7:;8:PDG-ODE-LER;9:;10:PDG-RBE-AQUAPOL-LDHP; C1 IFREMER, Lab Domesticat Huitre Perliere, Ctr Oceanol Pacifique, Taravao 98719, Tahiti, Fr Polynesia. IFREMER, Lab Biotechnol & Quake Perle, Ctr Oceanol Pacifique, Taravao 98719, Tahiti, Fr Polynesia. Univ Polynesie Francaise, Lab Biodiversite Terrestre & Marine, CNRS, UMR E44239, Faaa 98702, Tahiti, Fr Polynesia. Univ Paris 11, Lab Interact & Dynam Environm Surface, UMR IDES 8148, Orsay, France. IFREMER, Dept Environm Littoral & Ressources Aquacoles, F-29280 Plouzane, France. C2 IFREMER, FRANCE IFREMER, FRANCE UNIV POLYNESIE FRANCAISE, FRANCE UNIV PARIS 11, FRANCE IFREMER, FRANCE SI TAHITI BREST SE PDG-DOP-DCOP-AQUAPOL-LDHP PDG-RBE-AQUAPOL-LBQP PDG-RBE-PFOM-ARN PDG-RBE-AQUAPOL-LDHP PDG-ODE-LER IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.041 TC 33 UR https://archimer.ifremer.fr/doc/00028/13907/11195.pdf LA English DT Article DE ;Bivalvia;Pearl;Shell;Growth;Scanning electron microscope AB Marine mollusc shell growth has been widely measured using fluorochrome marking. In order to test the efficiency and reliability of calcein staining on P. margaritifera shells and pearls, the present study examined two administration methods, different concentrations and several immersion times.Immersion in a 150 mg.L-1 calcein solution for 12 h to 24 h appeared to be the best method for marking P. margaritifera shells. For pearl marking, injection of a 200 mg.L-1 calcein solution into the pearl pouch was the optimal method. Calcein marking was then used to measure the influence of food resource levels on the shell growth. Groups of 23-month-old P. margaritifera were fed at three trophic levels for two months. The two highest food levels tested (6 000 cell.mL-1 and 15 000 cell.mL-1) induced uniform growth between dorsal and ventral sides of shell, whereas the lowest food level (800 cell.mL-1) induced greater growth on the dorsal side. Shell deposits from the ventral side were observed using a scanning electron microscope, revealing that the difference of trophic level over two months had modified the thickness of the aragonite tablets formed. These results showed that trophic level is a major factor conditioning P. margaritifera development. PY 2011 PD MAR SO Aquaculture SN 0044-8486 PU Elsevier Science Bv VL 313 IS 1-4 UT 000289018500021 BP 149 EP 155 DI 10.1016/j.aquaculture.2011.01.008 ID 13907 ER EF