TY - JOUR T1 - Study on the microalgal pigments extraction process: Performance of microwave assisted extraction A1 - Pasquet,Virginie A1 - Cherouvrier,Jean-Rene A1 - Farhat,Firas A1 - Thiery,Valerie A1 - Piot,Jean-Marie A1 - Berard,Jean-Baptiste A1 - Kaas,Raymond A1 - Serive,Benoit A1 - Patrice,Thierry A1 - Cadoret,Jean-Paul A1 - Picot,Laurent AD - Univ La Rochelle, UMR CNRS LIENSs 6250, F-17042 La Rochelle, France. AD - Ctr IFREMER Nantes, IFREMER Lab PBA, F-44311 Nantes, France. AD - CHU Nantes, Dept LASER, F-44093 Nantes, France. UR - https://doi.org/10.1016/j.procbio.2010.07.009 DO - 10.1016/j.procbio.2010.07.009 KW - Pigments KW - Microalgae KW - Microwave KW - Extraction KW - Chlorophyll KW - Fucoxanthin KW - Carotene KW - MAE KW - VMAE N2 - The performance of microwaves irradiation (MAE and VMAE) to extract pigments from two marine microalgae was compared to conventional processes (cold and hot soaking and ultrasound-assisted extraction). Pigments were quantified by RP-HPLC and extraction performance was assessed regarding rapidity, reproducibility and extraction yields. Scanning electron microscopy was used at all extraction steps to assess the impact of the process on microalgal cell integrity. Freeze-drying and pigments extraction preserved microalgae cell integrity (except sonication) and evoked agglutination in superposed cells layers. All processes performed on Dunaliella tertiolecta (chlorophyte) lead to rapid pigments extraction, and equivalent pigments extraction yields, the absence of frustule allowing immediate solvent penetration in microalgae cells. In contrast, presence of the frustule in the diatom Cylindrotheca closterium (bacillariophyte) constituted a mechanical barrier to pigment extraction. MAE was identified as the best extraction process for CC pigments as it combined rapidity, reproducibility, homogeneous heating and high extraction yields. (C) 2010 Elsevier Ltd. All rights reserved. Y1 - 2011/01 PB - Elsevier Sci Ltd JF - Process Biochemistry SN - 1359-5113 VL - 46 IS - 1 SP - 59 EP - 67 ID - 14059 ER -