FN Archimer Export Format
PT J
TI Study on the microalgal pigments extraction process: Performance of microwave assisted extraction
BT 
AF PASQUET, Virginie
   CHEROUVRIER, Jean-Rene
   FARHAT, Firas
   THIERY, Valerie
   PIOT, Jean-Marie
   BERARD, Jean-Baptiste
   KAAS, Raymond
   SERIVE, Benoit
   PATRICE, Thierry
   CADORET, Jean-Paul
   PICOT, Laurent
AS 1:1;2:1;3:1;4:1;5:1;6:2;7:2;8:2;9:3;10:2;11:1;
FF 1:;2:;3:;4:;5:;6:PDG-DOP-DCN-BRM-PBA;7:PDG-DOP-DCN-BRM-PBA;8:PDG-DOP-DCN-BRM-PBA;9:;10:PDG-DOP-DCN-BRM-PBA;11:;
C1 Univ La Rochelle, UMR CNRS LIENSs 6250, F-17042 La Rochelle, France.
   Ctr IFREMER Nantes, IFREMER Lab PBA, F-44311 Nantes, France.
   CHU Nantes, Dept LASER, F-44093 Nantes, France.
C2 UNIV LA ROCHELLE, FRANCE
   IFREMER, FRANCE
   CHU NANTES, FRANCE
SI NANTES
SE PDG-DOP-DCN-BRM-PBA
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.627
TC 190
UR https://archimer.ifremer.fr/doc/00029/14059/11533.pdf
LA English
DT Article
DE ;Pigments;Microalgae;Microwave;Extraction;Chlorophyll;Fucoxanthin;Carotene;MAE;VMAE
AB The performance of microwaves irradiation (MAE and VMAE) to extract pigments from two marine microalgae was compared to conventional processes (cold and hot soaking and ultrasound-assisted extraction). Pigments were quantified by RP-HPLC and extraction performance was assessed regarding rapidity, reproducibility and extraction yields. Scanning electron microscopy was used at all extraction steps to assess the impact of the process on microalgal cell integrity. Freeze-drying and pigments extraction preserved microalgae cell integrity (except sonication) and evoked agglutination in superposed cells layers. All processes performed on Dunaliella tertiolecta (chlorophyte) lead to rapid pigments extraction, and equivalent pigments extraction yields, the absence of frustule allowing immediate solvent penetration in microalgae cells. In contrast, presence of the frustule in the diatom Cylindrotheca closterium (bacillariophyte) constituted a mechanical barrier to pigment extraction. MAE was identified as the best extraction process for CC pigments as it combined rapidity, reproducibility, homogeneous heating and high extraction yields. (C) 2010 Elsevier Ltd. All rights reserved.
PY 2011
PD JAN
SO Process Biochemistry
SN 1359-5113
PU Elsevier Sci Ltd
VL 46
IS 1
UT 000286538300007
BP 59
EP 67
DI 10.1016/j.procbio.2010.07.009
ID 14059
ER

EF