Mineral phase in shell repair of Manila clam Venerupis philippinarum affected by brown ring disease

Type Article
Date 2011-01
Language English
Author(s) Trinkler Nolwenn2, Bardeau Jean-Francois3, Marin Frederic4, 5, Labonne Maylis1, Jolivet Aurelie6, Crassous Philippe6, Paillard Christine2
Affiliation(s) 1 : CNRS, IRD, LEMAR, UMR UBO IRD 6539,Ctr IRD Bretagne, F-29280 Plouzane, France.
2 : CNRS, IUEM, LEMAR, UMR UBO IRD 6539, F-29280 Plouzane, France.
3 : Univ Maine, CNRS, LPEC, UMR 6087, F-72085 Le Mans 9, France.
4 : CNRS, UMR Biogeosci 5561, Dijon, France.
5 : Univ Bourgogne, F-21000 Dijon, France.
6 : IFREMER, STH, ZI Pointe du Diable, F-29280 Plouzane, France.
Source Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 2011-01 , Vol. 93 , N. 2 , P. 149-162
DOI 10.3354/dao02288
WOS© Times Cited 15
Keyword(s) Aragonite, Hemocytes, Shell microstructure, Calcium carbonate, Biomineralization, Mollusc, Raman microspectrometry, Scanning electron microscopy
Abstract The mineral phase of shell repair in the Manila clam Venerupis philippinarum affected by brown ring disease (BRD) was characterised at various scales and at various stages of shell repair by confocal Raman microspectrometry and scanning electron microscopy. Spherulitic and quadrangular aragonite microstructures associated with polyene pigments were clearly observed. Von Kossa staining showed that at the beginning of shell repair, hemocytes are filled with insoluble calcium carbonate salts in all fluids and then are transported toward the extrapallial fluids and the repair sites. Our analyses suggest that after a Vibrio tapetis attack and BRD deposit some clams rapidly cover the deposit, resulting in a modification in the microstructure, which could be produced by the participation of both the mantle and hemocytes.
Full Text
File Pages Size Access
Publisher's official version 14 3 MB Open access
Top of the page

How to cite 

Trinkler Nolwenn, Bardeau Jean-Francois, Marin Frederic, Labonne Maylis, Jolivet Aurelie, Crassous Philippe, Paillard Christine (2011). Mineral phase in shell repair of Manila clam Venerupis philippinarum affected by brown ring disease. Diseases Of Aquatic Organisms, 93(2), 149-162. Publisher's official version : https://doi.org/10.3354/dao02288 , Open Access version : https://archimer.ifremer.fr/doc/00029/14065/