FN Archimer Export Format PT J TI Mineral phase in shell repair of Manila clam Venerupis philippinarum affected by brown ring disease BT AF TRINKLER, Nolwenn BARDEAU, Jean-Francois MARIN, Frederic LABONNE, Maylis JOLIVET, Aurelie CRASSOUS, Philippe PAILLARD, Christine AS 1:2;2:3;3:4,5;4:1;5:6;6:6;7:2; FF 1:;2:;3:;4:;5:;6:PDG-DOP-DCB-EEP-LEP;7:; C1 CNRS, IRD, LEMAR, UMR UBO IRD 6539,Ctr IRD Bretagne, F-29280 Plouzane, France. CNRS, IUEM, LEMAR, UMR UBO IRD 6539, F-29280 Plouzane, France. Univ Maine, CNRS, LPEC, UMR 6087, F-72085 Le Mans 9, France. CNRS, UMR Biogeosci 5561, Dijon, France. Univ Bourgogne, F-21000 Dijon, France. IFREMER, STH, ZI Pointe du Diable, F-29280 Plouzane, France. C2 IRD, FRANCE UBO, FRANCE UNIV MAINE, FRANCE CNRS, FRANCE UNIV BOURGOGNE, FRANCE IFREMER, FRANCE SI BREST SE IRD PDG-DOP-DCB-EEP-LEP IUEM IN WOS Ifremer jusqu'en 2018 copubli-france copubli-p187 copubli-univ-france IF 2.201 TC 17 UR https://archimer.ifremer.fr/doc/00029/14065/11301.pdf LA English DT Article DE ;Aragonite;Hemocytes;Shell microstructure;Calcium carbonate;Biomineralization;Mollusc;Raman microspectrometry;Scanning electron microscopy AB The mineral phase of shell repair in the Manila clam Venerupis philippinarum affected by brown ring disease (BRD) was characterised at various scales and at various stages of shell repair by confocal Raman microspectrometry and scanning electron microscopy. Spherulitic and quadrangular aragonite microstructures associated with polyene pigments were clearly observed. Von Kossa staining showed that at the beginning of shell repair, hemocytes are filled with insoluble calcium carbonate salts in all fluids and then are transported toward the extrapallial fluids and the repair sites. Our analyses suggest that after a Vibrio tapetis attack and BRD deposit some clams rapidly cover the deposit, resulting in a modification in the microstructure, which could be produced by the participation of both the mantle and hemocytes. PY 2011 PD JAN SO Diseases Of Aquatic Organisms SN 0177-5103 PU Inter-research VL 93 IS 2 UT 000286611000007 BP 149 EP 162 DI 10.3354/dao02288 ID 14065 ER EF