Eutrophication in a tropical pond: Understanding the bacterioplankton and phytoplankton dynamics during a vibriosis outbreak using flow cytometric analyses

Type Article
Date 2010-12
Language English
Author(s) Lucas Ronan1, Courties C.2, 3, Herbland Alain1, Goulletquer PhilippeORCID4, Marteau Anne-Laure5, Lemonnier HuguesORCID1
Affiliation(s) 1 : IFREMER, Dept LEAD, Noumea 98846, New Caledonia.
2 : UPMC, Observ Oceanol, UMS 2348, F-66650 Banyuls Sur Mer, France.
3 : CNRS, Observ Oceanol, UMS 2348, F-66650 Banyuls Sur Mer, France.
4 : IFREMER, Direct Prospect & Strategie Sci, F-44311 Nantes, France.
5 : IAC, Paita 98890, New Caledonia.
Source Aquaculture (0044-8486) (Elsevier Science Bv), 2010-12 , Vol. 310 , N. 1-2 , P. 112-121
DOI 10.1016/j.aquaculture.2010.10.022
WOS© Times Cited 19
Keyword(s) Tropical shrimp aquaculture, Ecosystem shift, Phytoplankton, Bacterioplankton, Vibrio, Flow cytometry
Abstract In tropical shrimp ponds, the increasing of feed input, concomitantly with the stocking shrimp biomass, induces an eutrophication of the ecosystem. Although difficult to maintain, its stability is required to guarantee the success of the culture. A 110-day period of phytoplankton and bacterioplankton stock and dynamics in an earthen pond (1.2 ha area, 1 m depth) was monitored using flow cytometry to provide baseline information on community characteristics and ecosystem instability. Seven autotrophic cell types were identified over the whole sampling period. Prokaryotic cells included Synechococcus sp., a group named UNK which presented an atypical new flow cytometric signature and picoeukaryotes (PEUK). Nanophytoplankton cells were represented by 4 groups: NAN1, NAN2, NAN3 and Cryptophytes. During the first part of the survey, picophytoplankton dominated the phytoplanktonic assemblage. The mean abundance of total cells (up to 8 x 10(6) cells mL(-1)) was among the highest recorded in marine and brackish waters. Bacterial abundance and production ranged from 0.8 to 5.1 x 10(7) cells mL(-1) and from 30 to 110 mu g C L-1 h(-1). A shift from pico to nanophytoplankton abundance was observed for a few days from d 96. During this period, heterotrophic bacteria production and abundance suddenly dropped, implying a change in the functioning of the microbial loop. This shift was concomitant with a significant shrimp mortality outbreak due to Vibrio penaeicida, the etiological agent of a disease known as Syndrome 93, which affects the shrimp industry in New Caledonia. This survey suggests that flow cytometric analysis could be used for the monitoring of aquaculture systems to improve our understanding of the complex phytoplankton and bacterial dynamics of these systems and its potential influence on disease development. (c) 2010 Elsevier B.V. All rights reserved.
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