FN Archimer Export Format
PT J
TI Responses of diploid and triploid Pacific oysters Crassostrea gigas to Vibrio infection in relation to their reproductive status
BT 
AF DE DECKER, Sophie
   NORMAND, Julien
   SAULNIER, Denis
   PERNET, Fabrice
   CASTAGNET, Sophie
   BOUDRY, Pierre
AS 1:2;2:2;3:2;4:3;5:2;6:1;
FF 1:PDG-DOP-DCOP-AQNC;2:PDG-ODE-LER-LERN;3:PDG-RBE-AQUAPOL-LBQP;4:PDG-ODE-LER-LERLR;5:PDG-DOP-DCN-AGSAE-LGP;6:PDG-RBE-PFOM-PI;
C1 IFREMER, Lab Physiol Invertebres, F-29280 Plouzane, France.
   IFREMER, Lab Genet & Pathol, F-17390 Ronce Les Bains, La Tremblade, France.
   IFREMER, Lab Environm Ressources Languedoc Roussillon, F-34203 Sete, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI SAINT VINCENT
   PORT-EN-BESSIN
   TAHITI
   SETE
   LA TREMBLADE
   BREST
SE PDG-DOP-DCOP-AQNC
   PDG-ODE-LER-LERN
   PDG-RBE-AQUAPOL-LBQP
   PDG-ODE-LER-LERLR
   PDG-DOP-DCN-AGSAE-LGP
   PDG-RBE-PFOM-PI
IN WOS Ifremer jusqu'en 2018
IF 2.064
TC 33
UR https://archimer.ifremer.fr/doc/00030/14108/11368.pdf
LA English
DT Article
DE ;Experimental vibriosis;Crassostrea gigas;Vibrio;Survival distribution function;Triploidy;Reproductive effort
AB Several Vibrio species are known to be pathogenic to the Pacific oyster Crassostrea gigas. Survival varies according to pathogen exposure and high mortality events usually occur in summer during gametogenesis. In order to study the effects of gametogenetic status and ploidy (a factor known to affect reproduction allocation in oysters) on vibriosis survival, we conducted two successive experiments. Our results demonstrate that a common bath challenge with pathogenic Vibrio splendidus and Vibrio aestuarianus on a mixture of mature, spawning and non-mature oysters can lead to significant mortality. Previous bath challenges, which were done using only non-mature oysters, had not produced mortality. Immunohisto-chemical analyses showed the affinity of Vibrio for gonadic tissues, highlighting the importance of sexual maturity for vibriosis infection processes in oysters. Mortality rate results showed poor repeatability between tanks, however, in this bath challenge. We then tested a standardized and repeatable injection protocol using two different doses of the same combination of two Vibrio species on related diploid and triploid oysters at four different times over a year. Statistical analyses of mortality kinetics over a 6-day period after injection revealed that active gametogenesis periods correspond to higher susceptibility to vibriosis and that there is a significant interaction of this seasonal effect with ploidy. However, no significant advantage of triploidy was observed. Triploid oysters even showed lower survival than diploid counterparts in winter. Results are discussed in relation to differing energy allocation patterns between diploid and triploid Pacific oysters. (C) 2010 Elsevier Inc. All rights reserved.
PY 2011
PD FEB
SO Journal Of Invertebrate Pathology
SN 0022-2011
PU Academic Press Inc Elsevier Science
VL 106
IS 2
UT 000286701600003
BP 179
EP 191
DI 10.1016/j.jip.2010.09.003
ID 14108
ER

EF