FN Archimer Export Format
PT J
TI Use of OmpU porins for attachment and invasion of Crassostrea gigas immune cells by the oyster pathogen Vibrio splendidus
BT 
AF DUPERTHUY, Marylise
   SCHMITT, Paulina
   GARZON, Edwin
   CARO, Audrey
   ROSA, Rafael D.
   LE ROUX, Frederique
   LAUTREDOU-AUDOUY, Nicole
   GOT, Patrice
   ROMESTAND, Bernard
   DE LORGERIL, Julien
   KIEFFER-JAQUINOD, Sylvie
   BACHERE, Evelyne
   DESTOUMIEUX-GARZON, Delphine
AS 1:1,2,3,4;2:1,2,3,4;3:5;4:1,2,3,4;5:1,2,3,4;6:6,7;7:8,9;8:1,2,3,4;9:1,2,3,4;10:1,2,3,4;11:10;12:1,2,3,4;13:1,2,3,4;
FF 1:PDG-DOP-DCM-BOME-LALR;2:PDG-DOP-DCM-BOME-LALR;3:;4:;5:;6:PDG-RBE-PFOM;7:;8:;9:;10:PDG-RBE-BOME-LALR;11:;12:PDG-RBE-BOME;13:;
C1 Inst Francais Rech Exploitat Mer, F-34095 Montpellier, France.
   Ctr Natl Rech Sci, F-34095 Montpellier, France.
   Univ Montpellier 2, F-34095 Montpellier, France.
   Inst Rech Dev, Lab Ecosyst Lagunaires, Unite Mixte Rech 5119, F-34095 Montpellier, France.
   Ctr Cooperat Int Rech Agron Dev, Inst Rech Dev, Unite Mixte Rech 177, F-34094 Montpellier, France.
   CNRS, Biol Stn, F-29682 Roscoff, France.
   Inst Francais Rech Exploitat Mer, Lab Physiol Invertebres, F-29280 Brest, France.
   Ctr Natl Rech Sci, F-34090 Montpellier, France.
   Ctr Biochim Struct, Inst Natl Sante & Rech Med U554, Unite Mixte Rech 5048, F-34090 Montpellier, France.
   Univ Grenoble 1, Inst Natl Sante & Rech Med U880, Inst Rech Technol & Sci Vivant,Lab Etud Dynam Pro, Comm Energie Atom,Direct Sci Vivant, F-38054 Grenoble, France.
C2 IFREMER, FRANCE
   CNRS, FRANCE
   UNIV MONTPELLIER, FRANCE
   IRD, FRANCE
   CTR COOPERAT INT RECH AGRON DEV, FRANCE
   CNRS, FRANCE
   IFREMER, FRANCE
   CNRS, FRANCE
   CTR BIOCHIM STRUCT, FRANCE
   UNIV GRENOBLE, FRANCE
SI MONTPELLIER
   BREST
   PALAVAS
SE PDG-DOP-DCM-BOME-LALR
   PDG-RBE-PFOM
   PDG-RBE-BOME-LALR
   PDG-RBE-BOME
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-univ-france
IF 9.681
TC 117
UR https://archimer.ifremer.fr/doc/00031/14177/12166.pdf
LA English
DT Article
DE ;host-pathogen interaction;innate immunity;invertebrate;mollusk;oxidative burst
AB OmpU porins are increasingly recognized as key determinants of pathogenic host Vibrio interactions. Although mechanisms remain incompletely understood, various species, including the human pathogen Vibrio cholera, require OmpU for host colonization and virulence. We have shown previously that OmpU is essential for virulence in the oyster pathogen Vibrio splendidus LGP32. Here, we showed that V. splendidus LGP32 invades the oyster immune cells, the hemocytes, through subversion of host-cell actin cytoskeleton. In this process, OmpU serves as an adhesin/invasin required for beta-integrin recognition and host cell invasion. Furthermore, the major protein of oyster plasma, the extracellular superoxide dismutase Cg-EcSOD, is used as an opsonin mediating the OmpU-promoted phagocytosis through its RGD sequence. Finally, the endocytosed bacteria were found to survive intracellularly, evading the host defense by preventing acidic vacuole formation and limiting reactive oxygen species production. We conclude that (i) V. splendidus is a facultative intracellular pathogen that manipulates host defense mechanisms to enter and survive in host immune cells, and (ii) that OmpU is a major determinant of host cell invasion in Vibrio species, used by V. splendidus LGP32 to attach and invade oyster hemocytes through opsonisation by the oyster plasma Cg-EcSOD.
PY 2011
PD FEB
SO Proceedings Of The National Academy Of Sciences Of The United States Of America
SN 0027-8424
PU Natl Acad Sciences
VL 108
IS 7
UT 000287377000068
BP 2993
EP 2998
DI 10.1073/pnas.1015326108
ID 14177
ER

EF