Analytical Methods for Virus Detection in Water and Food

Type Article
Date 2011-03
Language English
Author(s) Bosch Albert1, 2, Sanchez Gloria3, Abbaszadegan Morteza4, Carducci Annalaura5, Guix Susana1, 2, Le Guyader Soizick6, Netshikweta Rembuluwani, Pinto Rosa M.1, 2, Van Der Poel Wim H. M.8, Rutjes Saskia9, Sano Daisuke1, 2, Taylor Maureen B.7, Van Zyl Walda B., Rodriguez-Lazaro David10, Kovac Katarina10, Sellwood Jane11
Affiliation(s) 1 : Univ Barcelona, Dept Microbiol, Enter Virus Lab, E-08028 Barcelona, Spain.
2 : Univ Barcelona, Inst Nutr & Food Safety INSA, E-08028 Barcelona, Spain.
3 : Inst Agrochem & Food Technol, Valencia, Spain.
4 : Arizona State Univ, Phoenix, AZ USA.
5 : Univ Pisa, Pisa, Italy.
6 : IFREMER, Nantes, France.
7 : Univ Pretoria, Natl Hlth Lab Serv Tshwane Acad Div, ZA-0002 Pretoria, South Africa.
8 : Univ Wageningen & Res Ctr, Cent Vet Inst, Lelystad, Netherlands.
9 : Natl Inst Publ Hlth & Environm RIVM, Bilthoven, Netherlands.
10 : ITACyL, Leon, Spain.
11 : British Stand Inst, Reading, Berks, England.
Source Food Analytical Methods (1936-9751) (Springer), 2011-03 , Vol. 4 , N. 1 , P. 4-12
DOI 10.1007/s12161-010-9161-5
WOS© Times Cited 88
Keyword(s) Enteric viruses, Gastroenteritis, Hepatitis, Detection, Concentration
Abstract Potential ways to address the issues that relate to the techniques for analyzing food and environmental samples for the presence of enteric viruses are discussed. It is not the authors' remit to produce or recommend standard or reference methods but to address specific issues in the analytical procedures. Foods of primary importance are bivalve molluscs, particularly, oysters, clams, and mussels; salad crops such as lettuce, green onions and other greens; and soft fruits such as raspberries and strawberries. All types of water, not only drinking water but also recreational water (fresh, marine, and swimming pool), river water (irrigation water), raw and treated sewage are potential vehicles for virus transmission. Well over 100 different enteric viruses could be food or water contaminants; however, with few exceptions, most well-characterized foodborne or waterborne viral outbreaks are restricted to hepatitis A virus (HAV) and calicivirus, essentially norovirus (NoV). Target viruses for analytical methods include, in addition to NoV and HAV, hepatitis E virus (HEV), enteroviruses (e.g., poliovirus), adenovirus, rotavirus, astrovirus, and any other relevant virus likely to be transmitted by food or water. A survey of the currently available methods for detection of viruses in food and environmental matrices was conducted, gathering information on protocols for extraction of viruses from various matrices and on the various specific detection techniques for each virus type.
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Bosch Albert, Sanchez Gloria, Abbaszadegan Morteza, Carducci Annalaura, Guix Susana, Le Guyader Soizick, Netshikweta Rembuluwani, Pinto Rosa M., Van Der Poel Wim H. M., Rutjes Saskia, Sano Daisuke, Taylor Maureen B., Van Zyl Walda B., Rodriguez-Lazaro David, Kovac Katarina, Sellwood Jane (2011). Analytical Methods for Virus Detection in Water and Food. Food Analytical Methods, 4(1), 4-12. Publisher's official version : , Open Access version :