Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels

Type Article
Date 2011-03
Language English
Author(s) Li Erchao1, 2, Arena Leticia3, Lizama Gabriel3, Gaxiola Gabriela3, Cuzon GerardORCID4, Rosas Carlos3, Chen Liqiao1, Van Wormhoudt Alain2
Affiliation(s) 1 : E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China.
2 : Museum Natl Hist Nat, Stn Biol Marine, UMR5178, F-29900 Concarneau, France.
3 : Univ Nacl Autonoma Mexico, Fac Ciencias, Unidad Multidisciplinaria Docencia & Invest, Hunucma 97350, Yucatan, Mexico.
4 : IFREMER, Tahiti, Fr Polynesia.
Source Chinese Journal Of Oceanology And Limnology (0254-4059) (Science Press), 2011-03 , Vol. 29 , N. 2 , P. 343-349
DOI 10.1007/s00343-011-0093-8
WOS© Times Cited 19
Keyword(s) Litopenaeus vannamei, gene expression, glutamate dehydrogenase, Na+-K+ ATPase, protein, salinity
Abstract Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp aquaculture. The effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp (L. vannamei) were investigated. This involved an examination of growth performance, glutamate dehydrogenase (GDH) and Na+-K+ ATPase mRNA expression,, and GDH activity in muscles and gills. Three experimental diets were formulated, containing 25%, 40%, and 50% dietary protein, and fed to the shrimp at a salinity of 25. After 20 days, no significant difference was observed in weight gain, though GDH and Na+-K+ ATPase gene expression and GDH activity increased with higher dietary protein levels. Subsequently, shrimp fed diets with 25% and 50% dietary protein were transferred into tanks with salinities of 38 and 5, respectively, and sampled at weeks 1 and 2. Shrimp fed with 40% protein at 25 in salinity (optimal conditions) were used as a control. Regardless of the salinities, shrimp fed with 50% dietary protein had significantly higher growth performance than other diets; no significant differences were found in comparison with the control. Shrimp fed with 25% dietary protein and maintained at salinities of 38 and 5 had significantly lower weight gain values after 2 weeks. Ambient salinity change also stimulated the hepatosomatic index, which increased in the first week and then recovered to a relatively normal level, as in the control, after 2 weeks. These findings indicate that in white shrimp, the specific protein nutrient and energy demands related to ambient salinity change are associated with protein metabolism. Increased dietary protein level could improve the osmoreaulation capacity of L. vannamei with more energy resources allocated to GDH activity and expression.
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Li Erchao, Arena Leticia, Lizama Gabriel, Gaxiola Gabriela, Cuzon Gerard, Rosas Carlos, Chen Liqiao, Van Wormhoudt Alain (2011). Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels. Chinese Journal Of Oceanology And Limnology, 29(2), 343-349. Publisher's official version : https://doi.org/10.1007/s00343-011-0093-8 , Open Access version : https://archimer.ifremer.fr/doc/00031/14261/