Osteoprotegerin, a new actor in vasculogenesis, stimulates endothelial colony-forming cells properties

Type Article
Date 2011-04
Language English
Author(s) Benslimane-Ahmim Z., Heymann D.2, 3, Dizier B., Lokajczyk A.4, Brion R.2, Laurendeau I.5, Bieche I.5, Smadja D. M.4, 6, Galy-Fauroux I.4, Colliec-Jouault SylviaORCID7, Fischer Anne-Marie4, 6, Boisson-Vidal Catherine1
Affiliation(s) 1 : Fac Pharm, INSERM, U765, F-75270 Paris 06, France.
2 : INSERM, UMR S 957, Nantes, France.
3 : Nantes Atlantique Univ, Univ Nantes, Nantes, France.
4 : Univ Paris Cite Paris Descartes, Fac Pharm, Paris, France.
5 : INSERM, U745, Genet Mol Lab, Paris, France.
6 : Hop Europeen Georges Pompidou, AP HP, Dept Haematol, Paris, France.
7 : IFREMER, Nantes, France.
Source Journal Of Thrombosis And Haemostasis (1538-7933) (Wiley-blackwell), 2011-04 , Vol. 9 , N. 4 , P. 834-843
DOI 10.1111/j.1538-7836.2011.04207.x
WOS© Times Cited 27
Keyword(s) endothelial colony-forming cells, osteoprotegerin, receptor activator of nuclear factor-kappa B ligand, vasculogenesis
Abstract Background: Osteoprotegerin (OPG), a soluble receptor of the tumour necrosis factor family, and its ligand, the receptor activator of nuclear factor-kappa B ligand (RANKL), are emerging as important regulators of vascular pathophysiology. Objectives: We evaluated their effects on vasculogenesis induced by endothelial colony-forming cells (ECFC) and on neovessel formation in vivo. Methods: Effects of OPG and RANKL on in vitro angiogenesis were evaluated after ECFC incubation with OPG or RANKL (0-50 ng mL(-1)). Effects on microvessel formation were evaluated with an in vivo murin Matrigel plug assay. Vascularization was evaluated by measuring plug hemoglobin and vascular endothelial growth factor (VEGF)-R2 content 14 days after implantation. Results: We found that ECFC expressed OPG and RANK but not RANKL mRNA. Treatment of ECFC with VEGF or stromal cell-derived factor-1 (SDF-1) upregulated OPG mRNA expression. OPG stimulated ECFC migration (P < 0.05), chemotaxis (P < 0.05) and vascular cord formation on Matrigel (R) (P < 0.01). These effects were correlated with SDF-1 mRNA overexpression, which was 30-fold higher after 4 h of OPG stimulation (P < 0.01). OPG-mediated angiogenesis involved the MAPK signaling pathway as well as Akt or mTOR cascades. RANKL also showed pro-vasculogenic effects in vitro. OPG combined with FGF-2 promoted neovessel formation in vivo, whereas RANKL had no effect. Conclusions: OPG induces ECFC activation and is a positive regulator of microvessel formation in vivo. Our results suggest that the OPG/RANK/RANKL axis may be involved in vasculogenesis and strongly support a modulatory role in tissue revascularization.
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Benslimane-Ahmim Z., Heymann D., Dizier B., Lokajczyk A., Brion R., Laurendeau I., Bieche I., Smadja D. M., Galy-Fauroux I., Colliec-Jouault Sylvia, Fischer Anne-Marie, Boisson-Vidal Catherine (2011). Osteoprotegerin, a new actor in vasculogenesis, stimulates endothelial colony-forming cells properties. Journal Of Thrombosis And Haemostasis, 9(4), 834-843. Publisher's official version : https://doi.org/10.1111/j.1538-7836.2011.04207.x , Open Access version : https://archimer.ifremer.fr/doc/00033/14462/