Archaeal Populations in Hypersaline Sediments Underlying Orange Microbial Mats in the Napoli Mud Volcano

Type Article
Date 2011-05
Language English
Author(s) Lazar Cassandre Sara1, L'Haridon Stephane1, Pignet Patricia2, Toffin LaurentORCID2
Affiliation(s) 1 : Univ Bretagne Occidentale, IFREMER, Ctr Brest,UMR 6197, Lab Microbiol Environm Extremes,Dept Etud Environ, F-29280 Plouzane, France.
Source Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 2011-05 , Vol. 77 , N. 9 , P. 3120-3131
DOI 10.1128/AEM.01296-10
WOS© Times Cited 16
Abstract Microbial mats in marine cold seeps are known to be associated with ascending sulfide- and methane-rich fluids. Hence, they could be visible indicators of anaerobic oxidation of methane (AOM) and methane cycling processes in underlying sediments. The Napoli mud volcano is situated in the Olimpi Area that lies on saline deposits; from there, brine fluids migrate upward to the seafloor. Sediments associated with a brine pool and microbial orange mats of the Napoli mud volcano were recovered during the Medeco cruise. Based on analysis of RNA-derived sequences, the "active" archaeal community was composed of many uncultured lineages, such as rice cluster V or marine benthic group D. Function methyl coenzyme M reductase (mcrA) genes were affiliated with the anaerobic methanotrophic Archaea (ANME) of the ANME-1, ANME-2a, and ANME-2c groups, suggesting that AOM occurred in these sediment layers. Enrichment cultures showed the presence of viable marine methylotrophic Methanococcoides in shallow sediment layers. Thus, the archaeal community diversity seems to show that active methane cycling took place in the hypersaline microbial mat-associated sediments of the Napoli mud volcano.
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