FN Archimer Export Format
PT J
TI Partial characterization of hepatopancreatic and extracellular digestive proteinases of wild and cultivated Octopus maya
BT 
AF MARTINEZ, Romain
   SANTOS, R.
   ALVAREZ, A.
   CUZON, Gerard
   ARENA, L.
   MASCARO, M.
   PASCUAL, C.
   ROSAS, C.
AS 1:5;2:4;3:3;4:2;5:1;6:1;7:1;8:1;
FF 1:;2:;3:;4:PDG-RBE-AQUAPOL;5:;6:;7:;8:;
C1 Univ Nacl Autonoma Mexico, Fac Ciencias, Unidad Multidisciplinaria Docencia & Invest, Sisal, Yucatan, Mexico.
   IFREMER, Tahiti, Fr Polynesia.
   UJAT, Unidad Ciencias Biol, Villahermosa, Tabasco, Mexico.
   Univ Autonoma Yucatan, FMVZ, Dept Nutr, Merida, Yucatan, Mexico.
   Univ Autonoma Yucatan, FMVZ, Div Posgrad, Merida, Yucatan, Mexico.
C2 UNIV UNAM, MEXICO
   IFREMER, FRANCE
   UNIV UJAT, MEXICO
   UNIV AUTONOMA YUCATAN, MEXICO
   UNIV AUTONOMA YUCATAN, MEXICO
SI BREST
   TAHITI
SE COFELY
   PDG-RBE-AQUAPOL
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
   copubli-sud
IF 0.912
TC 25
UR https://archimer.ifremer.fr/doc/00036/14683/17998.pdf
LA English
DT Article
DE ;Cephalopods;Digestive proteinases;Hepatopancreas;Gastric juice;Octopus maya
AB Proteinases from hepatopancreas (HP) and gastric juice (GJ) from wild and cultured red octopus (Octopus maya) were characterized. Hepatopancreas assays revealed optimal activity at pH 4, 9-10 and 10 for wild and pH 3, 8, and 9, for cultured octopuses, for total proteinases, trypsin and chymotrypsin, respectively. In the gastric juice, maximum activity was recorded at pH 6, 8, and 7 for total proteinases, trypsin, and chymotrypsin, respectively for both wild and cultured octopus. A reduction on enzyme activity of 70 and 20% was observed in HP and GJ extracts, respectively when protease inhibitor Pepstatin A was used. That result suggests that the main proteases in the HP were aspartic acid proteinases type (possibly Cathepsin D) and some of them were present in the GJ. Dissociating discontinuous polyacrylamide gel electrophoresis showed activity bands between 20 and 28, 30 and 34, 35 and 45, 60 and 70 kDa, and a last one between 75 and 100 kDa. We concluded that extracellular digestion of O. maya takes place in an acid environment, around pH 6. In contrast, intracellular digestion in the HP is developed at pHs between 3 and 4, where cathepsin D could be the most important enzyme for O. maya.
PY 2011
PD JUL
SO Aquaculture International
SN 0967-6120
PU Springer
VL 19
IS 3
UT 000290318300005
BP 445
EP 457
DI 10.1007/s10499-010-9360-5
ID 14683
ER

EF