FN Archimer Export Format
PT J
TI Strain-Dependent Norovirus Bioaccumulation in Oysters
BT 
AF MAALOUF, Haifa
   SCHAEFFER, Julien
   PARNAUDEAU, Sylvain
   LE PENDU, Jacques
   ATMAR, Robert L.
   CRAWFORD, Sue E.
   LE GUYADER, Soizick
AS 1:1;2:1;3:1;4:2;5:3;6:3;7:1;
FF 1:PDG-DOP-DCN-EMP-MIC;2:PDG-RBE-EMP-MIC;3:PDG-RBE-EMP-MIC;4:;5:;6:;7:PDG-RBE-EMP-MIC;
C1 IFREMER, Lab Microbiol LNR, F-44311 Nantes 03, France.
   Univ Nantes, INSERM, U892, Nantes, France.
   Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA.
C2 IFREMER, FRANCE
   UNIV NANTES, FRANCE
   BAYLOR COLL MED, USA
SI NANTES
SE PDG-DOP-DCN-EMP-MIC
   PDG-RBE-EMP-MIC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 3.829
TC 94
UR https://archimer.ifremer.fr/doc/00036/14753/12087.pdf
LA English
DT Article
AB Noroviruses (NoVs) are the main agents of gastroenteritis in humans and the primary pathogens of shellfish-related outbreaks. Some NoV strains bind to shellfish tissues by using carbohydrate structures similar to their human ligands, leading to the hypothesis that such ligands may influence bioaccumulation. This study compares the bioaccumulation efficiencies and tissue distributions in oysters (Crassostrea gigas) of three strains from the two principal human norovirus genogroups. Clear differences between strains were observed. The GI.1 strain was the most efficiently concentrated strain. Bioaccumulation specifically occurred in digestive tissues in a dose-dependent manner, and its efficiency paralleled ligand expression, which was highest during the cold months. In comparison, the GII.4 strain was very poorly bioaccumulated and was recovered in almost all tissues without seasonal influence. The GII.3 strain presented an intermediate behavior, without seasonal effect and with less bioaccumulation efficiency than that of the GI.1 strain during the cold months. In addition, the GII.3 strain was transiently concentrated in gills and mantle before being almost specifically accumulated in digestive tissues. Carbohydrate ligand specificities of the strains at least partly explain the strain-dependent bioaccumulation characteristics. In particular, binding to the digestive-tube-specific ligand should contribute to bioaccumulation, whereas we hypothesize that binding to the sialic acid-containing ligand present in all tissues would contribute to retain virus particles in the gills or mantle and lead to rapid destruction.
PY 2011
PD MAY
SO Applied And Environmental Microbiology
SN 0099-2240
PU Amer Soc Microbiology
VL 77
IS 10
UT 000290473200004
BP 3189
EP 3196
DI 10.1128/AEM.03010-10
ID 14753
ER

EF