FN Archimer Export Format
PT J
TI Experimental infection of Pacific oyster Crassostrea gigas spat by ostreid herpesvirus 1: demonstration of oyster spat susceptibility
BT 
AF SCHIKORSKI, David
   RENAULT, Tristan
   SAULNIER, Denis
   FAURY, Nicole
   MOREAU, Pierrick
   PEPIN, Jean-Francois
AS 1:1;2:1;3:1;4:1;5:1;6:1;
FF 1:PDG-RBE-AGSAE-LGP;2:PDG-RBE-AGSAE-LGP;3:PDG-RBE-AQUAPOL-LBQP;4:PDG-RBE-AGSAE-LGP;5:PDG-RBE-AGSAE-LGP;6:PDG-RBE-AGSAE-LGP;
C1 Ifremer Inst Francais Rech Exploitat Mer, LGP, F-17390 La Tremblade, France.
C2 IFREMER, FRANCE
SI BREST
   LA TREMBLADE
   TAHITI
SE PDG-RBE-AGSAE-LGP
   PDG-RBE-AQUAPOL-LBQP
IN WOS Ifremer jusqu'en 2018
IF 4.06
TC 129
UR https://archimer.ifremer.fr/doc/00037/14795/12104.pdf
LA English
DT Article
AB In 2008 and 2009, acute mortalities occurred in France among Pacific cupped oyster, Crassostrea gigas, spat. Different hypothesis including the implication of environmental factors, toxic algae and/or pathogens have been explored. Diagnostic tests indicated that OsHV-1 including a particular genotype, termed OsHV-1 mu Var, was detected in most of samples and especially in moribund oysters with the highlighting of virus particles looking like herpes viruses by TEM examination. In this study, an experimental protocol to reproduce OsHV-1 infection in laboratory conditions was developed. This protocol was based on the intramuscular injection of filtered (0.22 mu m) tissue homogenates prepared from naturally OsHV-1 infected spat collected on French coasts during mortality outbreaks in 2008. Results of the experimental trials showed that mortalities were induced after injection. Moreover, filtered tissue homogenates induced mortalities whereas the same tissue homogenates exposed to an ultraviolet (UV) treatment did not induce any mortality suggesting that oyster spat mortalities require the presence of a UV sensitive agent. Furthermore, analysis of injected oyster spat revealed the detection of high amounts of OsHV-1 DNA by real-time quantitative PCR. Finally, TEM analysis demonstrated the presence of herpes virus particles. The developed protocol allowed to maintain sources of infective virus which can be useful for the development of further studies concerning the transmission and the development of OsHV-1 infection.
PY 2011
PD FEB
SO Veterinary Research
SN 0928-4249
PU Biomed Central Ltd
VL 42
UT 000290660600007
BP 1
EP 13
DI 10.1186/1297-9716-42-27
ID 14795
ER

EF