|Author(s)||Vidal-Dupiol Jeremie, Ladriere Ophelie, Destoumieux Garzon Delphine, Sautiere Pierre-Eric, Meistertzheim Anne-Leila, Tambutte Eric, Tambutte Sylvie, Duval David, Foure Laurent, Adjeroud Mehdi, Mitta Guillaume|
|Affiliation(s)||Univ Perpignan Via Domitia, CNRS UPVD EPHE, UMR 5244, F-66000 Perpignan, France.NRS
Univ Liege, Unite Ecol Marine, Lab Ecol Anim & Ecotoxicol, B-4000 Liege, Belgium.
Univ Montpellier 2, CNRS, IFREMER, UMR 5119, F-34095 Montpellier, France.
Univ Lille 1, CNRS FRE 3249, IFR 147, Univ Lille Nord France, F-59655 Villeneuve Dascq, France.
Ctr Sci Monaco, MC-98000 Monaco, Monaco.
Aquarium Cap dAgde, F-34300 Cap Dagde, France.
Inst Rech Dev, Unite 227 CoReUs2, Noumea 98848, New Caledonia.
|Source||Journal Of Biological Chemistry (0021-9258) (Amer Soc Biochemistry Molecular Biology Inc), 2011-06 , Vol. 286 , N. 25 , P. 22688-22698|
|WOS© Times Cited||74|
|Abstract||Scleractinian corals are the most basal eumetazoan taxon and provide the biological and physical framework for coral reefs, which are among the most diverse of all ecosystems. Over the past three decades and coincident with climate change, these phototrophic symbiotic organisms have been subject to increasingly frequent and severe diseases, which are now geographically widespread and a major threat to these ecosystems. Although coral immunity has been the subject of increasing study, the available information remains fragmentary, especially with respect to coral antimicrobial responses. In this study, we characterized damicornin from Pocillopora damicornis, the first scleractinian antimicrobial peptide (AMP) to be reported. We found that its precursor has a segmented organization comprising a signal peptide, an acidic proregion, and the C-terminal AMP. The 40-residue AMP is cationic, C-terminally amidated, and characterized by the presence of six cysteine molecules joined by three intramolecular disulfide bridges. Its cysteine array is common to another AMP and toxins from cnidarians; this suggests a common ancestor, as has been proposed for AMPs and toxins from arthropods. Damicornin was active in vitro against Gram-positive bacteria and the fungus Fusarium oxysporum. Damicornin expression was studied using a combination of immunohistochemistry, reverse phase HPLC, and quantitative RT-PCR. Our data show that damicornin is constitutively transcribed in ectodermal granular cells, where it is stored, and further released in response to nonpathogenic immune challenge. Damicornin gene expression was repressed by the coral pathogen Vibrio coralliilyticus. This is the first evidence of AMP gene repression in a host-Vibrio interaction.|
Vidal-Dupiol Jeremie, Ladriere Ophelie, Destoumieux Garzon Delphine, Sautiere Pierre-Eric, Meistertzheim Anne-Leila, Tambutte Eric, Tambutte Sylvie, Duval David, Foure Laurent, Adjeroud Mehdi, Mitta Guillaume (2011). Innate Immune Responses of a Scleractinian Coral to Vibriosis. Journal Of Biological Chemistry, 286(25), 22688-22698. Publisher's official version : https://doi.org/10.1074/jbc.M110.216358 , Open Access version : https://archimer.ifremer.fr/doc/00039/14996/